The goal of this Program Project is to develop novel anti-tuberculosis vaccines that are safe and effective. Therefore, animal experiments involving virulent tuberculosis strains are a critical and essential component of the POl. The Animal Core, a state-of-the-art, well-staffed facility that strives to provide a safe, efficient, and cost-effective environment for the conduction of experiments using a variety of murine tuberculosis models, will provide invaluable services for animal studies proposed in each of the three projects of the Program Project proposal. The major goal ofthe Animal Core is to provide researchers ofthe Program Project with the technical support and training necessary for successful conduction of animal experiments that are requisite to successful development of novel vaccines. The PI and the staff of the Animal Core, who have extensive experience in modeling tuberculosis in mice, will also assist in experimental design. As a central program that services three individual components, the Animal Core can be expected to foster sharing of the resources available (for example, breeding and maintenance of specialty mice), stimulate scientific exchanges and collaboration, as well as train inexperienced young investigators interested in murine tuberculosis models. A substantial number of researchers inexperienced in animal experimentation have been trained to work in our biosafety level 3 Animal Core in the last funding period. In addition, physical expansion has let to doubling of the amount of space available for use by the Program Project investigators. Last but not least, many new pieces of equipment have been acquired including two Baker/Madison aerosolization machines that are currently fully operative, have greatly enhanced the efficiency of the facility. Based on the progress made in the last funding period, the Animal Core has clearly fulfilled its goals. We anticipate the Animal Core to continue to play an important role in facilitating animal experimentation for all the researchers who participate in the Program Project.
The Animal Core proposes to continue provide support, assistance, and training in conducting experiments that model tuberculosis in a variety mouse models. Development of safe and effective vaccines for human use necessitates the testing of candidates in animal models. This centralized facility is thus an essential component toward fulfilling this goal;and in addition, will likely shed considerable light on the events ensuing as a result of the interaction between the host and M. tuberculosis.
|Panas, Michael W; Sixsmith, Jaimie D; White, KeriAnn et al. (2014) Gene deletions in Mycobacterium bovis BCG stimulate increased CD8+ T cell responses. Infect Immun 82:5317-26|
|Chan, John; Mehta, Simren; Bharrhan, Sushma et al. (2014) The role of B cells and humoral immunity in Mycobacterium tuberculosis infection. Semin Immunol 26:588-600|
|Smith, Kristen L Jurcic; Saini, Divey; Bardarov, Svetoslav et al. (2014) Reduced virulence of an extensively drug-resistant outbreak strain of Mycobacterium tuberculosis in a murine model. PLoS One 9:e94953|
|Sixsmith, Jaimie D; Panas, Michael W; Lee, Sunhee et al. (2014) Recombinant Mycobacterium bovis bacillus Calmette-Guérin vectors prime for strong cellular responses to simian immunodeficiency virus gag in rhesus macaques. Clin Vaccine Immunol 21:1385-95|
|Carreño, Leandro J; Kharkwal, Shalu Sharma; Porcelli, Steven A (2014) Optimizing NKT cell ligands as vaccine adjuvants. Immunotherapy 6:309-20|
|Tufariello, JoAnn M; Malek, Adel A; Vilchèze, Catherine et al. (2014) Enhanced specialized transduction using recombineering in Mycobacterium tuberculosis. MBio 5:e01179-14|
|Prados-Rosales, Rafael; Carreño, Leandro J; Batista-Gonzalez, Ana et al. (2014) Mycobacterial membrane vesicles administered systemically in mice induce a protective immune response to surface compartments of Mycobacterium tuberculosis. MBio 5:e01921-14|
|Venkataswamy, Manjunatha M; Ng, Tony W; Kharkwal, Shalu S et al. (2014) Improving Mycobacterium bovis bacillus Calmette-Guèrin as a vaccine delivery vector for viral antigens by incorporation of glycolipid activators of NKT cells. PLoS One 9:e108383|
|Vilchèze, Catherine; Molle, Virginie; Carrère-Kremer, Séverine et al. (2014) Phosphorylation of KasB regulates virulence and acid-fastness in Mycobacterium tuberculosis. PLoS Pathog 10:e1004115|
|Kozakiewicz, Lee; Phuah, Jiayao; Flynn, Joanne et al. (2013) The role of B cells and humoral immunity in Mycobacterium tuberculosis infection. Adv Exp Med Biol 783:225-50|
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