The overall goal of the Immunology & Virology Core is to provide a centralized resource for detailed analysis of cellular and humoral immune responses for all investigators in this HIVRAD project. The Immunology & Virology Core will assist in the planning of assays, carry out assays of cellular and humoral immune responses for animals from multiple different projects, thus allowing accurate comparisons of immune responses among different experimental groups, serve as central repository for common reagents and experimental protocols, and assist in the interpretation and presentation of immunologic data. Specific assays that will be provided by the Immunology & Virology Core include standard and polychromatic flow cytometric analysis; analysis of cell-mediated immune responses using MHC tetramers, ELISPOT and intracellular cytokine staining; analysis of humoral immune responses using ELISA assays, Western blot and SIV neutralization assays; MHC class I typing by PCR; and preparation of virus stocks for challenge and neutralization assays.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program Projects (P01)
Project #
1P01AI071306-01A1
Application #
7294526
Study Section
Special Emphasis Panel (ZAI1-MPM-A (J1))
Project Start
2007-01-01
Project End
2012-03-31
Budget Start
2007-04-15
Budget End
2008-03-31
Support Year
1
Fiscal Year
2007
Total Cost
$503,291
Indirect Cost
Name
Harvard University
Department
Type
DUNS #
047006379
City
Boston
State
MA
Country
United States
Zip Code
02115
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