- MONOCLONAL CORE This Program Project addresses the impact of antibodies cross-reactive to DNA and the NMDAR, DNRAbs, on adult brain. The hypotheses to be tested are that NMDAR subunit composition, regional penetrance of antibody into brain parenchyma and antibody concentration determines the effect of the antibody on brain tissue and function, and that these antibodies can bind to cells not protected by the blood-brain barrier and alter cell function. Projects 1 and 3 both utilize a panel of monoclonal DNRAbs. These will need to be produced in large quantity. The Monoclonal Antibody Core will produce both human and murine antibodies and will test these for preservation of antigen-binding. The Core will produce antibody at high concentration using Hollow Fiber bioreactor, ascites or in miniPERM apparati. The IgG will be purified on Protein G columns. The Core will generate NR2A and NR2B extracellular domains for use in ELISAs to confirm antibody specificity. Project 2 requires that serum be tested for DNRAb titer. The Core will provide quality control for these assays also. This facility will be cost- effective and will maintain quality control for all 3 projects in the Program. This represents an efficient approach to the generation of reagents to be used by multiple investigators and one that maximizes the production of high quality reagents. Antibodies represent a highly versatile tool in medical research. They are used in diagnostics, in basic and clinical research and in developing new therapies. The DNRAbs created in our laboratory are utilized in all of the above areas not only in our studies but in many laboratories within the United States and abroad. They are used in studies evaluating their influence on the central nervous system and peripheral tissues, in studies revealing their role in neuropsychiatric lupus and in creating new therapies in SLE. Their utilization led to important medical observations and created a new area of studies in autoimmunity and in neurology.

Public Health Relevance

- MONOCLONAL CORE This Core will provide monoclonal antibodies and assessments of antibody titer for the studies in the Program Project and for laboratories world-wide that are interested in initiating basic or clinical studies of neuropsychiatric lupus. The studies enabled by these antibodies will hasten clinical strategies for neuroprotection in patients with lupus.

Agency
National Institute of Health (NIH)
Type
Research Program Projects (P01)
Project #
2P01AI073693-06A1
Application #
8741186
Study Section
Special Emphasis Panel (ZAI1)
Project Start
Project End
Budget Start
Budget End
Support Year
6
Fiscal Year
2014
Total Cost
Indirect Cost
Name
Feinstein Institute for Medical Research
Department
Type
DUNS #
City
Manhasset
State
NY
Country
United States
Zip Code
11030
Braniste, Viorica; Al-Asmakh, Maha; Kowal, Czeslawa et al. (2014) The gut microbiota influences blood-brain barrier permeability in mice. Sci Transl Med 6:263ra158
Vo, An; Volpe, Bruce T; Tang, Chris C et al. (2014) Regional brain metabolism in a murine systemic lupus erythematosus model. J Cereb Blood Flow Metab 34:1315-20
Chang, Eric H; Frattini, Stephen A; Robbiati, Sergio et al. (2013) Construction of microdrive arrays for chronic neural recordings in awake behaving mice. J Vis Exp :e50470
Cohen-Solal, J F G; Diamond, B (2011) [Neuropsychiatric lupus and autoantibodies against ionotropic glutamate receptor (NMDAR)]. Rev Med Interne 32:130-2
Cohen-Solal, Joel; Diamond, Betty (2011) Lessons from an anti-DNA autoantibody. Mol Immunol 48:1328-31
Bloom, Ona; Cheng, Kai Fan; Cheng, Kai Fen et al. (2011) Generation of a unique small molecule peptidomimetic that neutralizes lupus autoantibody activity. Proc Natl Acad Sci U S A 108:10255-9
Diamond, B; Bloom, O; Al Abed, Y et al. (2011) Moving towards a cure: blocking pathogenic antibodies in systemic lupus erythematosus. J Intern Med 269:36-44
Aranow, Cynthia; Diamond, Betty; Mackay, Meggan (2010) Glutamate receptor biology and its clinical significance in neuropsychiatric systemic lupus erythematosus. Rheum Dis Clin North Am 36:187-201, x-xi
Faust, Thomas W; Chang, Eric H; Kowal, Czeslawa et al. (2010) Neurotoxic lupus autoantibodies alter brain function through two distinct mechanisms. Proc Natl Acad Sci U S A 107:18569-74
Diamond, Betty (2010) Antibodies and the Brain: Lessons from Lupus. J Immunol 185:2637-2640

Showing the most recent 10 out of 15 publications