While highly active anti-retroviral therapy (HAART) can suppress HIV-1 replication, treatment currently does not eradicate infection or prevent a low level of continued replication. The major objective of this project is directed to defining the anatomic and cellular compartments in which HIV-1 is produced and persists before and during HAART, and from which infection re-emerges on discontinuation of HAART.
The Specific Aim of the project is to identify, characterize and quantify cellular sources of virus production and persistence in gutassociated lymphatic tissues (GALT) and peripheral lymphatic tissues (LTs), using state-of-the-art in situ Technologies of in situ hybridization, in situ PCR, immunohistochemistry and quantitative image analysis, with the long-term goal of capitalizing on the new insights into HIV-1 reservoirs generated by this analysis to design even more effective treatments.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program Projects (P01)
Project #
5P01AI074340-05
Application #
8379825
Study Section
Special Emphasis Panel (ZAI1-CCH-A)
Project Start
Project End
2014-08-31
Budget Start
2012-09-01
Budget End
2014-08-31
Support Year
5
Fiscal Year
2012
Total Cost
$253,059
Indirect Cost
$57,442
Name
University of Minnesota Twin Cities
Department
Type
DUNS #
555917996
City
Minneapolis
State
MN
Country
United States
Zip Code
55455
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