HIV infection is a major cause of morbidity and mortality among women worldwide. Few of the factors that increase susceptibility of women to HIV infection are known, and the development of woman-controlled HIV prevention methods is slow at best. We propose providing intensive logistical and field support of studies that link reproductive biology, HIV pathogenesis and pharmacology with regards to HIV. The goals of the Clinical and Data Core are to: 1) generate human subjects protocols, recruit, determine eligibility, and retain participants for program studies. 2) collect or obtain data, semen, cervical fluids and brushes, ecto- and endo-cervical and endometrial specimens for program studies according to each project's specifications, 3) measure tenofovir levels within cervical and endometrial tissues in the local Women's Interagency HIV Study (WIHS) participants of the WIHS pharmokinetics research, 4) obtain vaginal fluids from healthy women and women with bacterial vaginosis for studies of semen factors that influence male to female HIV transmission, 5) to interface with WIHS in conducting functional, transcriptome and immunohistochemistry studies of endometrial, ileal and sigmoid colon tissues that will be obtained by WIHS as part of its pathogenesis program. This application proposes a group of highly collaborative research projects that have intersecting data and specimen needs. Offering these resources via a single Core eliminates the need for redundant effort, ensures the efficient distribution of valuable samples, and frees laboratory-based investigators from supervising field activities. To the greatest extent possible use of existing data and specimens will be used, and when new recruitment is needed, we will endeavor to address the needs of multiple studies parsimoniously.

Public Health Relevance

The Clinical Core of this Research Program supports the research projects by providing access to human subjects, collecting tissues and blood specimens under the conditions determined by the project investigators, obtaining specific clinical measurements from human participants and tissues and supporting data processing, storage and analyses.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program Projects (P01)
Project #
5P01AI083050-05
Application #
8525321
Study Section
Special Emphasis Panel (ZAI1-TP-A)
Project Start
Project End
Budget Start
2013-09-01
Budget End
2014-08-31
Support Year
5
Fiscal Year
2013
Total Cost
$495,873
Indirect Cost
$79,287
Name
J. David Gladstone Institutes
Department
Type
DUNS #
099992430
City
San Francisco
State
CA
Country
United States
Zip Code
94158
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Zirafi, Onofrio; Kim, Kyeong-Ae; Roan, Nadia R et al. (2014) Semen enhances HIV infectivity and impairs the antiviral efficacy of microbicides. Sci Transl Med 6:262ra157
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