HIV infection is a major cause of morbidity and mortality among women worldwide. Few of the factors that increase susceptibility of women to HIV infection are known, and the development of woman-controlled HIV prevention methods is slow at best. We propose providing intensive logistical and field support of studies that link reproductive biology, HIV pathogenesis and pharmacology with regards to HIV. The goals of the Clinical and Data Core are to: 1) generate human subjects protocols, recruit, determine eligibility, and retain participants for program studies. 2) collect or obtain data, semen, cervical fluids and brushes, ecto- and endo-cervical and endometrial specimens for program studies according to each project's specifications, 3) measure tenofovir levels within cervical and endometrial tissues in the local Women's Interagency HIV Study (WIHS) participants of the WIHS pharmokinetics research, 4) obtain vaginal fluids from healthy women and women with bacterial vaginosis for studies of semen factors that influence male to female HIV transmission, 5) to interface with WIHS in conducting functional, transcriptome and immunohistochemistry studies of endometrial, ileal and sigmoid colon tissues that will be obtained by WIHS as part of its pathogenesis program. This application proposes a group of highly collaborative research projects that have intersecting data and specimen needs. Offering these resources via a single Core eliminates the need for redundant effort, ensures the efficient distribution of valuable samples, and frees laboratory-based investigators from supervising field activities. To the greatest extent possible use of existing data and specimens will be used, and when new recruitment is needed, we will endeavor to address the needs of multiple studies parsimoniously.

Public Health Relevance

The Clinical Core of this Research Program supports the research projects by providing access to human subjects, collecting tissues and blood specimens under the conditions determined by the project investigators, obtaining specific clinical measurements from human participants and tissues and supporting data processing, storage and analyses.

National Institute of Health (NIH)
National Institute of Allergy and Infectious Diseases (NIAID)
Research Program Projects (P01)
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Special Emphasis Panel (ZAI1-TP-A)
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J. David Gladstone Institutes
San Francisco
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Cavrois, Marielle; Neidleman, Jason; Santiago, Mario L et al. (2014) Enhanced fusion and virion incorporation for HIV-1 subtype C envelope glycoproteins with compact V1/V2 domains. J Virol 88:2083-94
Roan, Nadia R; Liu, Haichuan; Usmani, Shariq M et al. (2014) Liquefaction of semen generates and later degrades a conserved semenogelin peptide that enhances HIV infection. J Virol 88:7221-34
Zirafi, Onofrio; Kim, Kyeong-Ae; Roan, Nadia R et al. (2014) Semen enhances HIV infectivity and impairs the antiviral efficacy of microbicides. Sci Transl Med 6:262ra157
Chen, Joseph C; Johnson, Brittni A; Erikson, David W et al. (2014) Seminal plasma induces global transcriptomic changes associated with cell migration, proliferation and viability in endometrial epithelial cells and stromal fibroblasts. Hum Reprod 29:1255-70
Usmani, Shariq M; Zirafi, Onofrio; Müller, Janis A et al. (2014) Direct visualization of HIV-enhancing endogenous amyloid fibrils in human semen. Nat Commun 5:3508
Shanmugasundaram, Uma; Critchfield, J William; Pannell, Jane et al. (2014) Phenotype and functionality of CD4+ and CD8+ T cells in the upper reproductive tract of healthy premenopausal women. Am J Reprod Immunol 71:95-108
Chen, Joseph C; Erikson, David W; Piltonen, Terhi T et al. (2013) Coculturing human endometrial epithelial cells and stromal fibroblasts alters cell-specific gene expression and cytokine production. Fertil Steril 100:1132-43
Chan, Jonathan K L; Greene, Warner C (2011) NF-ýýB/Rel: agonist and antagonist roles in HIV-1 latency. Curr Opin HIV AIDS 6:12-8
Shacklett, Barbara L; Ferre, April L (2011) Mucosal immunity in HIV controllers: the right place at the right time. Curr Opin HIV AIDS 6:202-7
Shacklett, Barbara L; Greenblatt, Ruth M (2011) Immune responses to HIV in the female reproductive tract, immunologic parallels with the gastrointestinal tract, and research implications. Am J Reprod Immunol 65:230-41

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