It has only recently been recognized that bacteria, including the staphylococci, often exist as sessile organisms, attached to surfaces as highly complex and organized communities termed biofilms. These communifies are encased in matrices comprised of polysaccharides, proteins, and/or genomic DNA and are exposed to a remarkable variety of environmental conditions. Given that the study of bacterial biofilm is still relatively new, especially when considering staphylococcal biofllm, there exists a wide range of techniques and strategies to grow and analyze these complex structures. Not surprisingly, this variability makes it difficult to compare results from experiment to experiment and inhibits progress in understanding biofllm biology. To provide uniform and consistent strategies for the growth and analysis of biofilm, we propose a Core that will provide common biofllm growth equipment and analytical strategies for the participants of this PPG.
Specific Aim 1 of this Core will be to house and maintain equipment needed for biofilm growth and will include the purchase and maintenance of biofllm growth systems that will be extensively used by all four projects involved in this PPG. In addition, it will also maintain an anaerobic chamber for use in Projects 1 and 2.
Specific Aim will develop and maintain standardized protocols for biofilm growth and will serve to provide the protocols and expertise needed to produce consistent and reproducible biofllm results for all four Projects. Finally, Specific Aim 3 will be to acquire the technology needed for the analysis and impact of biofilm on the host. The goals of this aim will be to provide a microprobe detection system needed to detect fluctuations in speciflc metabolites produced during biofilm development, as well as a quanfitafive real-fime PCR system to measure changes in gene expression. It will also provide a Luminex Multi-analyte Detecfion system to follow the production of multiple inflammatory mediators during staphylococcal infection. Combined, these aims will make available the fundamental technology and expertise needed to carry-out all four projects described in this PPG and will help to ensure that reproducible results are obtained from project to project.

Public Health Relevance

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program Projects (P01)
Project #
5P01AI083211-05
Application #
8496683
Study Section
Special Emphasis Panel (ZAI1-TS-M)
Project Start
Project End
Budget Start
2013-07-01
Budget End
2014-06-30
Support Year
5
Fiscal Year
2013
Total Cost
$126,347
Indirect Cost
$35,195
Name
University of Nebraska Medical Center
Department
Type
DUNS #
168559177
City
Omaha
State
NE
Country
United States
Zip Code
68198
Yamada, Kelsey J; Kielian, Tammy (2018) Biofilm-Leukocyte Cross-Talk: Impact on Immune Polarization and Immunometabolism. J Innate Immun :1-9
Bhinderwala, Fatema; Lonergan, Samantha; Woods, Jade et al. (2018) Expanding the Coverage of the Metabolome with Nitrogen-Based NMR. Anal Chem 90:4521-4528
Heim, Cortney E; Vidlak, Debbie; Odvody, Jessica et al. (2018) Human prosthetic joint infections are associated with myeloid-derived suppressor cells (MDSCs): Implications for infection persistence. J Orthop Res 36:1605-1613
Svechkarev, Denis; Sadykov, Marat R; Bayles, Kenneth W et al. (2018) Ratiometric Fluorescent Sensor Array as a Versatile Tool for Bacterial Pathogen Identification and Analysis. ACS Sens 3:700-708
Yamada, Kelsey J; Heim, Cortney E; Aldrich, Amy L et al. (2018) Arginase-1 Expression in Myeloid Cells Regulates Staphylococcus aureus Planktonic but Not Biofilm Infection. Infect Immun 86:
King, Alyssa N; Borkar, Samiksha; Samuels, David J et al. (2018) Guanine limitation results in CodY-dependent and -independent alteration of Staphylococcus aureus physiology and gene expression. J Bacteriol :
Mlynek, Kevin D; Sause, William E; Moormeier, Derek E et al. (2018) Nutritional Regulation of the Sae Two-Component System by CodY in Staphylococcus aureus. J Bacteriol 200:
Gries, Casey M; Kielian, Tammy (2017) Staphylococcal Biofilms and Immune Polarization During Prosthetic Joint Infection. J Am Acad Orthop Surg 25 Suppl 1:S20-S24
Krute, Christina N; Rice, Kelly C; Bose, Jeffrey L (2017) VfrB Is a Key Activator of the Staphylococcus aureus SaeRS Two-Component System. J Bacteriol 199:
Moormeier, Derek E; Bayles, Kenneth W (2017) Staphylococcus aureus biofilm: a complex developmental organism. Mol Microbiol 104:365-376

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