An essential aspect of this PPG is to maintain a state-of-the-art core facility that supports the imaging needs of the four proposed projects. Similar to the work performed in the initial funding cycle, we propose to make use of a variety of microscopic and macroscopic imaging technologies to gain a better understanding of biofilm assembly and metabolism, and how these affect the host immune response both in vitro and in vivo. Thus, the primary function of this Bioimaging Core will be to maintain the cutting-edge instrumentation needed to visualize the specific transcriptional and metabolic changes that occur during biofilm development, and to assess the impact of these changes in the host environment. The first specific aim of this core is to upgrade and maintain the Center for Staphylococcal Research (CSR) confocal microscopy facility.
This aim will provide an upgrade for the existing Zeiss LSM510 META confocal microscope utilized by all members of our group to the more powerful LSM710 series, thus, providing optimal imaging capabilities. The second specific aim will be to provide the maintenance and expertise needed to analyze biofilm development using our BioFlux Microfluidics System.
This aim will be primarily utilized by Projects 1 and 3 to assess the effects of various metabolic and regulatory mutations on biofilm development and gene expression. The third and final specific aim will be to support our In Vivo Imaging System (IVIS) Spectrum instrument, which will be used by Projects 2, 3, and 4 to visualize the progression of infection in live animals. All three of these aims will provide essential technology needed to successfully execute the goals of the projects described in this proposal.
|Ibberson, Carolyn B; Parlet, Corey P; Kwiecinski, Jakub et al. (2016) Hyaluronan Modulation Impacts Staphylococcus aureus Biofilm Infection. Infect Immun 84:1917-29|
|Marshall, Darrell D; Sadykov, Marat R; Thomas, Vinai C et al. (2016) Redox Imbalance Underlies the Fitness Defect Associated with Inactivation of the Pta-AckA Pathway in Staphylococcus aureus. J Proteome Res 15:1205-12|
|Kavanaugh, Jeffrey S; Horswill, Alexander R (2016) Impact of Environmental Cues on Staphylococcal Quorum Sensing and Biofilm Development. J Biol Chem 291:12556-64|
|Gries, Casey M; Sadykov, Marat R; Bulock, Logan L et al. (2016) Potassium Uptake Modulates Staphylococcus aureus Metabolism. mSphere 1:|
|Windham, Ian H; Chaudhari, Sujata S; Bose, Jeffrey L et al. (2016) SrrAB Modulates Staphylococcus aureus Cell Death through Regulation of cidABC Transcription. J Bacteriol 198:1114-22|
|Chaudhari, Sujata S; Thomas, Vinai C; Sadykov, Marat R et al. (2016) The LysR-type transcriptional regulator, CidR, regulates stationary phase cell death in Staphylococcus aureus. Mol Microbiol 101:942-53|
|Vidlak, Debbie; Kielian, Tammy (2016) Infectious Dose Dictates the Host Response during Staphylococcus aureus Orthopedic-Implant Biofilm Infection. Infect Immun 84:1957-65|
|Paharik, Alexandra E; Horswill, Alexander R (2016) The Staphylococcal Biofilm: Adhesins, Regulation, and Host Response. Microbiol Spectr 4:|
|Schaeffer, Carolyn R; Hoang, Tra-My N; Sudbeck, Craig M et al. (2016) Versatility of Biofilm Matrix Molecules in Staphylococcus epidermidis Clinical Isolates and Importance of Polysaccharide Intercellular Adhesin Expression during High Shear Stress. mSphere 1:|
|Lewis, April M; Rice, Kelly C (2016) Quantitative Real-Time PCR (qPCR) Workflow for Analyzing Staphylococcus aureus Gene Expression. Methods Mol Biol 1373:143-54|
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