An essential aspect of this PPG is to maintain a state-of-the-art core facility that supports the imaging needs of the four proposed projects. Similar to the work performed in the initial funding cycle, we propose to make use of a variety of microscopic and macroscopic imaging technologies to gain a better understanding of biofilm assembly and metabolism, and how these affect the host immune response both in vitro and in vivo. Thus, the primary function of this Bioimaging Core will be to maintain the cutting-edge instrumentation needed to visualize the specific transcriptional and metabolic changes that occur during biofilm development, and to assess the impact of these changes in the host environment. The first specific aim of this core is to upgrade and maintain the Center for Staphylococcal Research (CSR) confocal microscopy facility.
This aim will provide an upgrade for the existing Zeiss LSM510 META confocal microscope utilized by all members of our group to the more powerful LSM710 series, thus, providing optimal imaging capabilities. The second specific aim will be to provide the maintenance and expertise needed to analyze biofilm development using our BioFlux Microfluidics System.
This aim will be primarily utilized by Projects 1 and 3 to assess the effects of various metabolic and regulatory mutations on biofilm development and gene expression. The third and final specific aim will be to support our In Vivo Imaging System (IVIS) Spectrum instrument, which will be used by Projects 2, 3, and 4 to visualize the progression of infection in live animals. All three of these aims will provide essential technology needed to successfully execute the goals of the projects described in this proposal.

National Institute of Health (NIH)
Research Program Projects (P01)
Project #
Application #
Study Section
Special Emphasis Panel (ZAI1)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of Nebraska Medical Center
United States
Zip Code
Lehman, McKenzie K; Bose, Jeffrey L; Sharma-Kuinkel, Batu K et al. (2015) Identification of the amino acids essential for LytSR-mediated signal transduction in Staphylococcus aureus and their roles in biofilm-specific gene expression. Mol Microbiol 95:723-37
Hanamsagar, Richa; Aldrich, Amy; Kielian, Tammy (2014) Critical role for the AIM2 inflammasome during acute CNS bacterial infection. J Neurochem 129:704-11
Scherr, Tyler D; Lindgren, Kevin E; Schaeffer, Carolyn R et al. (2014) Mouse model of post-arthroplasty Staphylococcus epidermidis joint infection. Methods Mol Biol 1106:173-81
Heim, Cortney E; Vidlak, Debbie; Scherr, Tyler D et al. (2014) Myeloid-derived suppressor cells contribute to Staphylococcus aureus orthopedic biofilm infection. J Immunol 192:3778-92
Hernandez, Frank J; Huang, Lingyan; Olson, Michael E et al. (2014) Noninvasive imaging of Staphylococcus aureus infections with a nuclease-activated probe. Nat Med 20:301-6
Zurek, Oliwia W; Nygaard, Tyler K; Watkins, Robert L et al. (2014) The role of innate immunity in promoting SaeR/S-mediated virulence in Staphylococcus aureus. J Innate Immun 6:21-30
Kiedrowski, Megan R; Crosby, Heidi A; Hernandez, Frank J et al. (2014) Staphylococcus aureus Nuc2 is a functional, surface-attached extracellular nuclease. PLoS One 9:e95574
Sapp, April M; Mogen, Austin B; Almand, Erin A et al. (2014) Contribution of the nos-pdt operon to virulence phenotypes in methicillin-sensitive Staphylococcus aureus. PLoS One 9:e108868
Olson, Michael E; Todd, Daniel A; Schaeffer, Carolyn R et al. (2014) Staphylococcus epidermidis agr quorum-sensing system: signal identification, cross talk, and importance in colonization. J Bacteriol 196:3482-93
Lindgren, J K; Thomas, V C; Olson, M E et al. (2014) Arginine deiminase in Staphylococcus epidermidis functions to augment biofilm maturation through pH homeostasis. J Bacteriol 196:2277-89

Showing the most recent 10 out of 48 publications