The Histopathology Core will provide integral support for all 3 projects. This core will centralize the pathology resources for all of the projects of the PPG. This will result in cost savings, increased quality control and maximal comparability between the results of the projects. Key functions of the Core include: 1) expert advise in immunohistological aspects of experimental design to optimize results;2) uniform tissue processing;3) tissue sectioning (frozen sections and paraffin-embedded sections);4) histochemical and immunohistochemical staining of tissue sections;5) pathological interpretation of tissue sections;6) documentation of the pathology by high quality digital microscopic photography;7) preparation of figures for manuscripts;8) storage and retrieval of stained slides for each of the projects. In addition, this Core will be responsible for ordering, quality assurance and control studies of all antibodies and reagents for use in the pathology methods. New reagents will be developed and evaluated as requested by investigators in the 3 projects.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program Projects (P01)
Project #
5P01AI087586-04
Application #
8470541
Study Section
Special Emphasis Panel (ZAI1-PTM-I)
Project Start
Project End
Budget Start
2013-06-01
Budget End
2014-05-31
Support Year
4
Fiscal Year
2013
Total Cost
$218,938
Indirect Cost
$79,487
Name
Cleveland Clinic Lerner
Department
Type
DUNS #
135781701
City
Cleveland
State
OH
Country
United States
Zip Code
44195
Yagisawa, Takafumi; Tanaka, Toshiaki; Miyairi, Satoshi et al. (2018) In the absence of natural killer cell activation donor-specific antibody mediates chronic, but not acute, kidney allograft rejection. Kidney Int :
Iida, Shoichi; Miyairi, Satoshi; Su, Charles A et al. (2018) Peritransplant VLA-4 blockade inhibits endogenous memory CD8 T cell infiltration into high-risk cardiac allografts and CTLA-4Ig resistant rejection. Am J Transplant :
Tsuda, Hidetoshi; Su, Charles A; Tanaka, Toshiaki et al. (2018) Allograft dendritic cell p40 homodimers activate donor-reactive memory CD8+ T cells. JCI Insight 3:
Ayasoufi, Katayoun; Kohei, Naoki; Nicosia, Michael et al. (2018) Aquaporin 4 blockade improves survival of murine heart allografts subjected to prolonged cold ischemia. Am J Transplant 18:1238-1246
Danturti, Sreedevi; Keslar, Karen S; Steinhoff, Leah R et al. (2017) CD4+ T lymphocytes produce adiponectin in response to transplants. JCI Insight 2:
Purroy, Carolina; Fairchild, Robert L; Tanaka, Toshiaki et al. (2017) Erythropoietin Receptor-Mediated Molecular Crosstalk Promotes T Cell Immunoregulation and Transplant Survival. J Am Soc Nephrol 28:2377-2392
Benichou, Gilles; Gonzalez, Bruno; Marino, Jose et al. (2017) Role of Memory T Cells in Allograft Rejection and Tolerance. Front Immunol 8:170
Iida, Shoichi; Tsuda, Hidetoshi; Tanaka, Toshiaki et al. (2016) IL-1 Receptor Signaling on Graft Parenchymal Cells Regulates Memory and De Novo Donor-Reactive CD8 T Cell Responses to Cardiac Allografts. J Immunol 196:2827-37
Gorbacheva, Victoria; Fan, Ran; Fairchild, Robert L et al. (2016) Memory CD4 T Cells Induce Antibody-Mediated Rejection of Renal Allografts. J Am Soc Nephrol 27:3299-3307
Kohei, Naoki; Tanaka, Toshiaki; Tanabe, Kazunari et al. (2016) Natural killer cells play a critical role in mediating inflammation and graft failure during antibody-mediated rejection of kidney allografts. Kidney Int 89:1293-306

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