The development of an effective AIDS vaccine remains one of the highest priorities in HIV research. Studies of HIV pathogenesis, vaccine design and antiretroviral treatments require the use of an appropriate animal model. However, the currently used SIV/rhesus macaque (RM) model has serious limitations. The overall objective of this HIVRAD proposal is identify and characterize novel, moleculariy defined, physiologically relevant SIVsmm strains that reproducibly lead to productive infection in RMs and more faithfully recapitulate the genetic diversity and the clinical outcome of currently circulating HIV-1 strains. These new challenge strains will also be used to provide new mechanistic insight into mucosal HIV/SIV transmission. The Nonhuman Primate Core located at the University of Pittsburgh will be responsible for managing a large portion of the primate studies required for this Program Projects.
The Specific Aims i nclude: 1. To select, monitor and manage all non-human primates required by Projects 1 and 2. The goals are to provide these projects with the appropriate animals for the proposed studies, to ensure high quality animal housing and general husbandry, and to perform all aspects of the clinical management of the animals, including the care, treatment and long-term monitoring. 2. To coordinate the design and scheduling of non-human primate studies within the HIVRAD consortium. The goals are to coordinate the experimental design, arrange for efficient scheduling, collect, process, store and inventory all samples from RMs, and distribute the samples to the research groups. 3. To perform all necropsies required by Projects 1 and 2. The goals are to arrange for tissue collection, processing and storage, perform mononuclear cell separation from blood, non-lymphoid and lymphoid tissues and conduct all histopathological examinations. The purpose of the Animal Core Component is to provide high quality of animal care and housing, execution of study protocols, specimen collection and processing, performance of necropsies, and acquisition and management of animal clinical and pathological data necessary to meet the program research objectives. Dr. Pandrea and the Core team will closely interact with Project leaders and their laboratory staff to ensure timely completion of all NHP related research goals.
Results from these HIVRAD studies will shed new light on the molecular mechanisms underiying mucosal HIV/SIV transmission, identify new targets for vaccine-elicited immune responses, and generate a much needed set of new genetically-diverse SIVsmm/mac challenge strains for transmission, pathogenesis and AIDS vaccine research.
|Smedley, Jeremy; Turkbey, Baris; Bernardo, Marcelino L et al. (2014) Tracking the luminal exposure and lymphatic drainage pathways of intravaginal and intrarectal inocula used in nonhuman primate models of HIV transmission. PLoS One 9:e92830|
|Ma, Dongzhu; Jasinska, Anna J; Feyertag, Felix et al. (2014) Factors associated with siman immunodeficiency virus transmission in a natural African nonhuman primate host in the wild. J Virol 88:5687-705|
|Roederer, Mario; Keele, Brandon F; Schmidt, Stephen D et al. (2014) Immunological and virological mechanisms of vaccine-mediated protection against SIV and HIV. Nature 505:502-8|
|Ma, Dongzhu; Jasinska, Anna; Kristoff, Jan et al. (2013) SIVagm infection in wild African green monkeys from South Africa: epidemiology, natural history, and evolutionary considerations. PLoS Pathog 9:e1003011|
|Lopker, Michael; Easlick, Juliet; Sterrett, Sarah et al. (2013) Heterogeneity in neutralization sensitivities of viruses comprising the simian immunodeficiency virus SIVsmE660 isolate and vaccine challenge stock. J Virol 87:5477-92|
|Parrish, Nicholas F; Gao, Feng; Li, Hui et al. (2013) Phenotypic properties of transmitted founder HIV-1. Proc Natl Acad Sci U S A 110:6626-33|
|Liao, Hua-Xin; Lynch, Rebecca; Zhou, Tongqing et al. (2013) Co-evolution of a broadly neutralizing HIV-1 antibody and founder virus. Nature 496:469-76|
|Liao, Hua-Xin; Tsao, Chun-Yen; Alam, S Munir et al. (2013) Antigenicity and immunogenicity of transmitted/founder, consensus, and chronic envelope glycoproteins of human immunodeficiency virus type 1. J Virol 87:4185-201|
|Vaccari, Monica; Keele, Brandon F; Bosinger, Steven E et al. (2013) Protection afforded by an HIV vaccine candidate in macaques depends on the dose of SIVmac251 at challenge exposure. J Virol 87:3538-48|
|Kong, Rui; Li, Hui; Bibollet-Ruche, Frederic et al. (2012) Broad and potent neutralizing antibody responses elicited in natural HIV-2 infection. J Virol 86:947-60|
Showing the most recent 10 out of 11 publications