The purpose of the Animal and Microsurgery Core (Core B) is to establish a focused facility that provides investigators in each of the two projects with sufficient breeding of animals with given genotypes and with a standardized cardiac transplantation and tolerance induction procedure for the proposed experiments. Core B will provide a key service role that will enable the Principal Investigators and their staff to focus on experimental and scientific efforts. Centralized breeding and screening of the progeny will ensure that animals of required genotypes are available for experiments. Transplantation performed by a centralized source will standardize the procedures, minimize surgically imposed disparities in the grafts used by the two projects and allow some long-term acceptors generated for Project 1 (studying the induction of tolerance) to be used for Project 2 (studying the maintenance of tolerance) to minimize experimental cost. In addition, purchases of reagents such as anesthetics and immunosuppressants will be done in bulk for both projects to reduce cost. The specific tasks of Core B will be to: a) breed and genotype animals to be used for both projects;b) transplant heart allografts and perform thymectomies as directed by the project leaders and their personnel;c) prepare and inject donor splenocytes and administer indicated immunosuppressive therapies;d) monitor the beating of the transplanted hearts, the health of the transplant recipients, and record times of rejection;e) sacrifice transplanted animals as directed by the Principal Investigators and their personnel and collect organs for histology, as well as to distribute to other project personnel for phenotypic and functional analysis of immune cells.
(Relevance) The Animal and Microsurgery Core (Core B) will serve as a centralize source to provide animal breeding and genotyping as well as cardiac transplantation and thymectomy to generate experimental animals to be used by the 2 projects. Protocols will be standardized to minimize disparities between the 2 projects.
|Chong, Anita S (2016) From Pipe Dream to Donor-Specific PC Elimination: Novel Ways to Target Alloantibodies. Transplantation 100:2238-2239|
|Lei, Yuk Man; Chen, Luqiu; Wang, Ying et al. (2016) The composition of the microbiota modulates allograft rejection. J Clin Invest 126:2736-44|
|Alegre, Maria-Luisa; Lakkis, Fadi G; Morelli, Adrian E (2016) Antigen Presentation in Transplantation. Trends Immunol 37:831-843|
|Miller, Michelle L; Chen, Jianjun; Daniels, Melvin D et al. (2016) Adoptive transfer of tracer alloreactive CD4(+) TCR-transgenic T cells alters the endogenous immune response to an allograft. Am J Transplant :|
|Molinero, Luciana L; Yin, Dengping; Lei, Yuk Man et al. (2016) High-Fat Diet-Induced Obesity Enhances Allograft Rejection. Transplantation 100:1015-21|
|Yang, Jinghui; Chen, Jianjun; Young, James S et al. (2016) Tracing Donor-MHC Class II Reactive B cells in Mouse Cardiac Transplantation: Delayed CTLA4-Ig Treatment Prevents Memory Alloreactive B-Cell Generation. Transplantation 100:1683-91|
|Young, J S; Chen, J; Miller, M L et al. (2016) Delayed Cytotoxic T Lymphocyte-Associated Protein 4-Immunoglobulin Treatment Reverses Ongoing Alloantibody Responses and Rescues Allografts From Acute Rejection. Am J Transplant 16:2312-23|
|Chen, Jianjun; Wang, Qiang; Yin, Dengping et al. (2015) Cutting Edge: CTLA-4Ig Inhibits Memory B Cell Responses and Promotes Allograft Survival in Sensitized Recipients. J Immunol 195:4069-73|
|Bartman, Caroline; Chong, Anita S; Alegre, Maria-Luisa (2015) The influence of the microbiota on the immune response to transplantation. Curr Opin Organ Transplant 20:1-7|
|Geissler, Edward K; Tullius, Stefan G; Chong, Anita S (2015) Establishment of a global virtual laboratory for transplantation: a symposium report. Transplantation 99:381-4|
Showing the most recent 10 out of 21 publications