Core B supports every project within the Program by providing access to state-of-the-art RNAi tools. Short hairpin RNAs (shRNAs) were developed with the support of this program, and ongoing innovations by Core B and Program investigators have helped to make these extremely powerful biological tools. During the past funding period, the Core devised methods to rapidly engineer mice carrying regulated shRNA expression cassettes, devised strategies for functionally validating shRNAs in a multiplexed fashion, and created a third generation of RNAi libraries corresponding to annotated protein coding genes in humans and mice. During the upcoming period of requested support, the core proposes to aid Program investigators through five general aims. First, the program will continue to provide access to state-of-the-art RNAi tools for analyzing either single gene knockdowns or for performing pooled RNAi screens. Second, the Core will work with investigators to operate the """"""""sensor assay"""""""" to identify optimal shRNAs tools against genes of interest. Third, the core will produce custom RNAi libraries against sets of genes that are of interest to Program investigators. Fourth, the Core will produce mice carrying regulated RNAi cassettes and aid investigators in combining these with other desired genetic lesions. Finally, the Core will carry on its efforts to improve RNAi technologies and make those innovations available to the Program and to the community at large.

Public Health Relevance

RNAi has become a mainstay of modern biology. Core B and investigators within this program project have continued to be world leaders in the development of shRNAs as powerful experimental tools. Thus, work within the Core impacts not only the Program but also the broader community.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Program Projects (P01)
Project #
5P01CA013106-43
Application #
8744363
Study Section
Special Emphasis Panel (ZCA1-RPRB-0)
Project Start
Project End
Budget Start
2014-01-01
Budget End
2014-12-31
Support Year
43
Fiscal Year
2014
Total Cost
$322,337
Indirect Cost
$154,321
Name
Cold Spring Harbor Laboratory
Department
Type
DUNS #
065968786
City
Cold Spring Harbor
State
NY
Country
United States
Zip Code
11724
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