Core A provides Project researchers with state-of-the-art analytical tools, techniques and experience in the characterization and quantitation of chemical substances and modifications of cellular molecules such as DNA, RNA, lipids, proteins and small-molecule metabolites. It also provides well-characterized cell lines and controlled delivery of nitric-oxide-related DNA and protein damaging agents to these cells to insure quality-control and reproducibility among the Projects. It operates as a service, a developmental laboratory, and as a resource for the Program. Individual researchers use the facilities as needed at three levels: as a service lab, for supervised analyses, or as fully trained users. In addition, we continually expand and improve our capabilities with the development and acquisition of technologies that facilitate the research programs.
Specific aims evolve yearly and typically include the development and improvement of Project-related analytical methods, evaluation of new hardware, and evaluation of recently-acquired or potentially useful software. For the year just ending, these were the development and improvement of project-related analytical methods (e.g., quantitation of modified tyrosines), exploitation evaluation of new hardware, and continued focus on targeted quantitation of peptides and proteins, e.g., using click-chemistry-based derivatization for sample enrichment. New equipment and expertise needs are identified in member surveys conducted by the Administrative Core and one-on-one discussions with Project leaders, and via Program- Project group meetings.
Inflammation is now recognized as a major factor in the etiology of many types of cancer. The proposed research will open new understanding of the role of the chemical changes to proteins and DNA brought about by the immune cells attracted to the site of inflammation. The overall chemical damage contributes strongly to the process leading to cancer.
|Whary, Mark T; Muthupalani, Sureshkumar; Ge, Zhongming et al. (2014) Helminth co-infection in Helicobacter pylori infected INS-GAS mice attenuates gastric premalignant lesions of epithelial dysplasia and glandular atrophy and preserves colonization resistance of the stomach to lower bowel microbiota. Microbes Infect 16:345-55|
|Kiraly, Orsolya; Gong, Guanyu; Roytman, Megan D et al. (2014) DNA glycosylase activity and cell proliferation are key factors in modulating homologous recombination in vivo. Carcinogenesis 35:2495-502|
|Lertpiriyapong, Kvin; Handt, Laurence; Feng, Yan et al. (2014) Pathogenic properties of enterohepatic Helicobacter spp. isolated from rhesus macaques with intestinal adenocarcinoma. J Med Microbiol 63:1004-16|
|Ge, Zhongming; Feng, Yan; Muthupalani, Sureshkumar et al. (2014) Helicobacter hepaticus cholesterol-?-glucosyltransferase is essential for establishing colonization in male A/JCr mice. Helicobacter 19:280-8|
|Shrivastav, Nidhi; Fedeles, Bogdan I; Li, Deyu et al. (2014) A chemical genetics analysis of the roles of bypass polymerase DinB and DNA repair protein AlkB in processing N2-alkylguanine lesions in vivo. PLoS One 9:e94716|
|Fox, James G; Wang, Timothy C (2014) Dietary factors modulate Helicobacter-associated gastric cancer in rodent models. Toxicol Pathol 42:162-81|
|Kumar, Yadunanda; Liang, Cui; Limmon, Gino V et al. (2014) Molecular analysis of serum and bronchoalveolar lavage in a mouse model of influenza reveals markers of disease severity that can be clinically useful in humans. PLoS One 9:e86912|
|Singh, Vipender; Peng, Chunte Sam; Li, Deyu et al. (2014) Direct observation of multiple tautomers of oxythiamine and their recognition by the thiamine pyrophosphate riboswitch. ACS Chem Biol 9:227-36|
|Li, Deyu; Fedeles, Bogdan I; Singh, Vipender et al. (2014) Tautomerism provides a molecular explanation for the mutagenic properties of the anti-HIV nucleoside 5-aza-5,6-dihydro-2'-deoxycytidine. Proc Natl Acad Sci U S A 111:E3252-9|
|Swennes, Alton G; Sheh, Alexander; Parry, Nicola M A et al. (2014) Helicobacter hepaticus infection promotes hepatitis and preneoplastic foci in farnesoid X receptor (FXR) deficient mice. PLoS One 9:e106764|
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