Core A takes care of the tissue processing needs for all projects. It is directly supervised by Dr. Pelayo Correa and his assistant Dr. M. Blanca Piazuelo. In Nashville our personnel receive all biopsies from Colombia. Biopsies for histopathology evaluation are fixed immediately in buffered zinc-formalin, embedded in paraffin, sectioned and stained with hematoxylin eosin and modified Warthin- Starry silver stain. Biopsies for frozen sections are received in OCT and frozen immediately. The laboratory performs all immunohistochemical stains, including Ki67, TUNEL assays, inducible nitric oxide synthase, nitrotyrosine and a variety of white blood cell markers. Fully trained and experienced personnel are available for all procedures.

Public Health Relevance

The damage done by infection to the stomach lining is examined by taking small pieces (biopsies) of the mucosa with the gastroscope. These biopsies are processed by this core to be examined under the microscope by the investigators

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Program Projects (P01)
Project #
5P01CA028842-28
Application #
8540096
Study Section
Special Emphasis Panel (ZCA1-GRB-P)
Project Start
Project End
Budget Start
2013-09-01
Budget End
2014-08-31
Support Year
28
Fiscal Year
2013
Total Cost
$115,281
Indirect Cost
$38,846
Name
Vanderbilt University Medical Center
Department
Type
DUNS #
004413456
City
Nashville
State
TN
Country
United States
Zip Code
37212
Garay, Jone; Piazuelo, M Blanca; Majumdar, Sumana et al. (2016) The homing receptor CD44 is involved in the progression of precancerous gastric lesions in patients infected with Helicobacter pylori and in development of mucous metaplasia in mice. Cancer Lett 371:90-8
Murray-Stewart, T; Sierra, J C; Piazuelo, M B et al. (2016) Epigenetic silencing of miR-124 prevents spermine oxidase regulation: implications for Helicobacter pylori-induced gastric cancer. Oncogene 35:5480-5488
Yang, Ines; Woltemate, Sabrina; Piazuelo, M Blanca et al. (2016) Different gastric microbiota compositions in two human populations with high and low gastric cancer risk in Colombia. Sci Rep 6:18594
Sobota, Rafal S; Kodaman, Nuri; Mera, Robertino et al. (2016) Epigenetic and genetic variation in GATA5 is associated with gastric disease risk. Hum Genet 135:895-906
Esmail, Michael Y; Bacon, Rebecca; Swennes, Alton G et al. (2016) Helicobacter Species Identified in Captive Sooty Mangabeys (Cercocebus atys) with Metastatic Gastric Adenocarcinoma. Helicobacter 21:175-85
Camargo, M Constanza; Kim, Kyoung-Mee; Matsuo, Keitaro et al. (2016) Anti-Helicobacter pylori Antibody Profiles in Epstein-Barr virus (EBV)-Positive and EBV-Negative Gastric Cancer. Helicobacter 21:153-7
Dubeykovskaya, Zina; Si, Yiling; Chen, Xiaowei et al. (2016) Neural innervation stimulates splenic TFF2 to arrest myeloid cell expansion and cancer. Nat Commun 7:10517
Singh, Kshipra; Al-Greene, Nicole T; Verriere, Thomas G et al. (2016) The L-Arginine Transporter Solute Carrier Family 7 Member 2 Mediates the Immunopathogenesis of Attaching and Effacing Bacteria. PLoS Pathog 12:e1005984
Dixon, Beverly R E A; Radin, Jana N; Piazuelo, M Blanca et al. (2016) IL-17a and IL-22 Induce Expression of Antimicrobials in Gastrointestinal Epithelial Cells and May Contribute to Epithelial Cell Defense against Helicobacter pylori. PLoS One 11:e0148514
Hardbower, Dana M; Asim, Mohammad; Murray-Stewart, Tracy et al. (2016) Arginase 2 deletion leads to enhanced M1 macrophage activation and upregulated polyamine metabolism in response to Helicobacter pylori infection. Amino Acids :

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