Core B will provide support for all of the animal experiments in Projects 1 and 2 of the proposal. It includes oversight of quality control of ali aspects of animal purchases, maintenance of the animal colony, breeding of mice, UM Unit for Laboratory Animal Medicine regulatory communications, experimental transplantation of mice, and histopathological analysis. It is designed to enhance harmonization and efficiency of the experiments that utilize animals in Projects 1 and 2. The services provided by Core B are: 1. To oversee and monitor adherence to the standards required by the University of Michigan Unit for Laboratory Animal Medicine (ULAM) 2. To facilitate centralized and orderly animal purchases and provide for routine but uniform pretransplant and post-BMT animal care and specialized breeding ofthe knock-out mice 3. To prepare donor cell inoculums and perform BMT following irradiation in a standardized manner 4. To provide supervision for consistent scoring of clinical GVHD in the transplant models 5. To provide standardized harvesting of target organs, processing, staining (hematoxylin and eosin) and shipping of samples for histopathological analysis by Dr. Chen Liu at the University of Florida 6. To provide uniform and consistent histologic analysis of GVHD pathology in target organ tissues

Public Health Relevance

Allogeneic hematopoietic stem cell transplantation is a potentially curative therapy for many malignant diseases whose applicability has been impeded by the development of its most serious complication, GVHD. Strategies that mitigate GVHD will allow for better harnessing of this effective therapeutic modality to treat many patients with hematological cancers.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Program Projects (P01)
Project #
5P01CA039542-26
Application #
8545543
Study Section
Special Emphasis Panel (ZCA1-RPRB-J)
Project Start
Project End
Budget Start
2013-09-01
Budget End
2014-08-31
Support Year
26
Fiscal Year
2013
Total Cost
$309,596
Indirect Cost
$87,438
Name
University of Michigan Ann Arbor
Department
Type
DUNS #
073133571
City
Ann Arbor
State
MI
Country
United States
Zip Code
48109
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