The purpose ofthis core Is to provide a cen tialized laboratory resource for cryopresen/ation and distiibution of patient samples to program investigators. The core virtll also maintain an annotated database with relevant pathologic data for each sample and develop genomic and functional assays to support clinical investigation. This core has 4 Specific Aims: 1. To acquire primary leukemia samples from p atients ente red in clinical research protocols and to isolate and preserve RNA, DNA, and viable mononuclear cells. 2. To maintain an annotated database containing pathologic, cytogenetic and molecular infomiation for clinical samples obtained from patients witii myeloid leukemias. 3. To develop a set of standardized genomic and functional assays to support clinteal investigation. 4. To maintain a bank of AML cell lines optimized for in vivo studies and a bank of AML

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This core will be responsible for obtaining blood and marrow samples from patients with myel old leukemia and maintain a repository virith Vn ese leukemia samples. Patient confkjentiality vM be maintained and samples will only be ob tained from patients who have provided inform ed consent. These samples will be provkled to program investigators for research studies outlined in each projecl This core will also use these samples to tievelop and carry out genetic and functional assays.

National Institute of Health (NIH)
National Cancer Institute (NCI)
Research Program Projects (P01)
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Special Emphasis Panel (ZCA1-RPRB-C (J1))
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Brigham and Women's Hospital
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Hanoun, Maher; Zhang, Dachuan; Mizoguchi, Toshihide et al. (2014) Acute myelogenous leukemia-induced sympathetic neuropathy promotes malignancy in an altered hematopoietic stem cell niche. Cell Stem Cell 15:365-75
Adamia, Sophia; Bar-Natan, Michal; Haibe-Kains, Benjamin et al. (2014) NOTCH2 and FLT3 gene mis-splicings are common events in patients with acute myeloid leukemia (AML): new potential targets in AML. Blood 123:2816-25
Heckl, Dirk; Kowalczyk, Monika S; Yudovich, David et al. (2014) Generation of mouse models of myeloid malignancy with combinatorial genetic lesions using CRISPR-Cas9 genome editing. Nat Biotechnol 32:941-6
Adamia, Sophia; Haibe-Kains, Benjamin; Pilarski, Patrick M et al. (2014) A genome-wide aberrant RNA splicing in patients with acute myeloid leukemia identifies novel potential disease markers and therapeutic targets. Clin Cancer Res 20:1135-45
Bruedigam, Claudia; Bagger, Frederik O; Heidel, Florian H et al. (2014) Telomerase inhibition effectively targets mouse and human AML stem cells and delays relapse following chemotherapy. Cell Stem Cell 15:775-90
Santos, Margarida A; Faryabi, Robert B; Ergen, Aysegul V et al. (2014) DNA-damage-induced differentiation of leukaemic cells as an anti-cancer barrier. Nature 514:107-11
Schneider, Rebekka K; Ademà, Vera; Heckl, Dirk et al. (2014) Role of casein kinase 1A1 in the biology and targeted therapy of del(5q) MDS. Cancer Cell 26:509-20
Liu, Suiyang; Yin, Li; Stroopinsky, Dina et al. (2014) MUC1-C oncoprotein promotes FLT3 receptor activation in acute myeloid leukemia cells. Blood 123:734-42
Liss, Adam; Ooi, Chia-Huey; Zjablovskaja, Polina et al. (2014) The gene signature in CCAAT-enhancer-binding protein * dysfunctional acute myeloid leukemia predicts responsiveness to histone deacetylase inhibitors. Haematologica 99:697-705
Ng, C E L; Sinha, A; Krivtsov, A et al. (2014) KRas(G12D)-evoked leukemogenesis does not require *-catenin. Leukemia 28:698-702

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