The utilization of genetically engineered mouse (GEIVI) strains is an integral component of this GTBT P01 program project. The mouse model Core will provide all the necessary expertise, reagents and services for the analysis of exosomal biology with regards to therapeutic applications in GEM models of GBM. The services and reagents provided by this Core will synergize with the individual Projects on the validation and assessment of exosomal-associated molecular biomarkers and the application of cutting edge monitoring technologies for tumor response to therapeutic agents. In addition, the Core will produce and characterize three new GBM GEM strains over the funding period. As such, the Core will support all aspects of the construction of these new transgenic mouse models, including advice, service, technologies and reagents for the optimal design and construction of each specific strain. Using these new strains, the Core will also evaluate and implement new technologies for the detection and monitoring of various exosomal metrics in close relationship with the individual Projects.

Public Health Relevance

The goal of this Core is to provide technical and intellectual support for the use of genetically engineered mouse models of GBM as a conduit for monitoring tumor growth response in relation to therapeutic intervention using exosome-based detection and content analysis

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Program Projects (P01)
Project #
5P01CA069246-17
Application #
8657805
Study Section
Special Emphasis Panel (ZCA1-GRB-P)
Project Start
Project End
Budget Start
2014-05-01
Budget End
2015-04-30
Support Year
17
Fiscal Year
2014
Total Cost
$249,154
Indirect Cost
$58,366
Name
Massachusetts General Hospital
Department
Type
DUNS #
073130411
City
Boston
State
MA
Country
United States
Zip Code
02199
Rooj, Arun K; Ricklefs, Franz; Mineo, Marco et al. (2017) MicroRNA-Mediated Dynamic Bidirectional Shift between the Subclasses of Glioblastoma Stem-like Cells. Cell Rep 19:2026-2032
Choudhury, Sourav R; Hudry, Eloise; Maguire, Casey A et al. (2017) Viral vectors for therapy of neurologic diseases. Neuropharmacology 120:63-80
Maas, Sybren L N; Breakefield, Xandra O; Weaver, Alissa M (2017) Extracellular Vesicles: Unique Intercellular Delivery Vehicles. Trends Cell Biol 27:172-188
Speranza, Maria-Carmela; Passaro, Carmela; Ricklefs, Franz et al. (2017) Preclinical investigation of gene-mediated cytotoxic immunotherapy and checkpoint blockade in glioblastoma. Neuro Oncol :
Min, Changwook; Park, Jongmin; Mun, Jae Kyoung et al. (2017) Integrated microHall magnetometer to measure the magnetic properties of nanoparticles. Lab Chip 17:4000-4007
Yeo, Alan T; Charest, Alain (2017) Immune Checkpoint Blockade Biology in Mouse Models of Glioblastoma. J Cell Biochem 118:2516-2527
Im, Hyungsoon; Lee, Kyungheon; Weissleder, Ralph et al. (2017) Novel nanosensing technologies for exosome detection and profiling. Lab Chip 17:2892-2898
Godlewski, Jakub; Ferrer-Luna, Ruben; Rooj, Arun K et al. (2017) MicroRNA Signatures and Molecular Subtypes of Glioblastoma: The Role of Extracellular Transfer. Stem Cell Reports 8:1497-1505
Wei, Zhiyun; Batagov, Arsen O; Schinelli, Sergio et al. (2017) Coding and noncoding landscape of extracellular RNA released by human glioma stem cells. Nat Commun 8:1145
Zappulli, Valentina; Friis, Kristina Pagh; Fitzpatrick, Zachary et al. (2016) Extracellular vesicles and intercellular communication within the nervous system. J Clin Invest 126:1198-207

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