This program project examines the structure, biosynthesis, and roles of carbohydrates in cell-cell interaction. The proposed projects were borne-out from our strong collaborative research activities in the previous funding period. This program project is a continued close collaboration among investigators whose expertise ranges from carbohydrate synthetic chemistry, carbohydrate biosynthesis, immunology, to cellular and molecular biology. In the coming years, we will add strength in research capability by incorporating intravital microscopy to observe migrating and interacting cells in vivo. The work centers around sulfated, fucosylated glycoconjugates in lymphocyte trafficking, tumor progression, and myelopoiesis. The work includes the role of carbohydrate-mediated signal transduction, a novel aspect in the field, through studies on the role of fucose in Notch signaling. The work also determines the role of carbohydrate-dependent tumor metastasis by identifying novel carbohydrate recognition molecules and their use for tumor targeting, and determining the roles of heparan sulfate in tumor angiogenesis. These studies will provide new information on the roles of carbohydrates in myelopoiesis, chronic inflammation, tumor progression, and carbohydrate-dependent signal transduction. The knowledge gained from these studies may help in the design of improved therapeutic intervention against malignancies and other diseases.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Program Projects (P01)
Project #
5P01CA071932-15
Application #
8308594
Study Section
Special Emphasis Panel (ZCA1-RPRB-O (M1))
Program Officer
Sathyamoorthy, Neeraja
Project Start
1997-05-01
Project End
2014-07-31
Budget Start
2012-08-01
Budget End
2014-07-31
Support Year
15
Fiscal Year
2012
Total Cost
$1,395,034
Indirect Cost
$464,947
Name
Sanford-Burnham Medical Research Institute
Department
Type
DUNS #
020520466
City
La Jolla
State
CA
Country
United States
Zip Code
92037
Nonaka, Motohiro; Fukuda, Michiko N; Gao, Chao et al. (2014) Determination of carbohydrate structure recognized by prostate-specific F77 monoclonal antibody through expression analysis of glycosyltransferase genes. J Biol Chem 289:16478-86
Nonaka, Motohiro; Bao, Xingfeng; Matsumura, Fumiko et al. (2014) Synthetic di-sulfated iduronic acid attenuates asthmatic response by blocking T-cell recruitment to inflammatory sites. Proc Natl Acad Sci U S A 111:8173-8
Sugihara, Kazuhiro; Shibata, Toshiaki K; Takata, Kayoko et al. (2013) Attenuation of fibroblast growth factor signaling by poly-N-acetyllactosamine type glycans. FEBS Lett 587:3195-201
Lee, Seung Ho; Fukuda, Minoru (2013) Study of the biological functions of mucin type core 3 O-glycans. Methods Mol Biol 1022:41-50
Tsuboi, Koichiro; Hirakawa, Jotaro; Seki, Emiko et al. (2013) Role of high endothelial venule-expressed heparan sulfate in chemokine presentation and lymphocyte homing. J Immunol 191:448-55
Suzuki-Anekoji, Misa; Suzuki, Atsushi; Wu, Sz-Wei et al. (2013) In vivo regulation of steroid hormones by the Chst10 sulfotransferase in mouse. J Biol Chem 288:5007-16
Okamoto, Teppei; Yoneyama, Mihoko Sutoh; Hatakeyama, Shingo et al. (2013) Core2 O-glycan-expressing prostate cancer cells are resistant to NK cell immunity. Mol Med Rep 7:359-64
Pols, Maaike S; van Meel, Eline; Oorschot, Viola et al. (2013) hVps41 and VAMP7 function in direct TGN to late endosome transport of lysosomal membrane proteins. Nat Commun 4:1361
Ito, Yuki; Vela, Jose Luis; Matsumura, Fumiko et al. (2013) Helicobacter pylori cholesteryl ?-glucosides contribute to its pathogenicity and immune response by natural killer T cells. PLoS One 8:e78191
Hatakeyama, Shingo; Shibata, Toshiaki K; Tobisawa, Yuki et al. (2013) Tumor targeting by a carbohydrate ligand-mimicking peptide. Methods Mol Biol 1022:369-86

Showing the most recent 10 out of 161 publications