Abstract

The facility intends to synthesize 2 grams of oligomer per year for the Program. The oligomer including 1 gram S-DNA, 700 mg normal DNA and 300 mg RNA and special modified oligos. With the installation of the second Expedite Synthesizer we should be able to offer oligonucleotides at costs that will remain stable in the next 4-5 years. The facility's Technical Director, Dr. Xiaolin Zhang, is responsible for quality control of the DNA produced daily.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Program Projects (P01)
Project #
3P01CA072765-05S1
Application #
6491049
Study Section
Project Start
2001-07-16
Project End
2003-06-30
Budget Start
Budget End
Support Year
5
Fiscal Year
2001
Total Cost
Indirect Cost

  Institution

Name
University of Pennsylvania
Department
Type
DUNS #
042250712
City
Philadelphia
State
PA
Country
United States
Zip Code
19104

  Publications

Kini, Hemant K; Kong, Jian; Liebhaber, Stephen A (2014) Cytoplasmic poly(A) binding protein C4 serves a critical role in erythroid differentiation. Mol Cell Biol 34:1300-9
Ji, Xinjun; Wan, Ji; Vishnu, Melanie et al. (2013) ?CP Poly(C) binding proteins act as global regulators of alternative polyadenylation. Mol Cell Biol 33:2560-73
Ji, Xinjun; Kong, Jian; Liebhaber, Stephen A (2011) An RNA-protein complex links enhanced nuclear 3' processing with cytoplasmic mRNA stabilization. EMBO J 30:2622-33
Jin, Shenghao; Zhao, Huiwu; Yi, Yan et al. (2010) c-Myb binds MLL through menin in human leukemia cells and is an important driver of MLL-associated leukemogenesis. J Clin Invest 120:593-606
Deleavey, Glen F; Watts, Jonathan K; Alain, Tommy et al. (2010) Synergistic effects between analogs of DNA and RNA improve the potency of siRNA-mediated gene silencing. Nucleic Acids Res 38:4547-57
Waggoner, Shelly A; Johannes, Gregg J; Liebhaber, Stephen A (2009) Depletion of the poly(C)-binding proteins alphaCP1 and alphaCP2 from K562 cells leads to p53-independent induction of cyclin-dependent kinase inhibitor (CDKN1A) and G1 arrest. J Biol Chem 284:9039-49
Flagler, K; Alexeev, V; Pierce, E A et al. (2008) Site-specific gene modification by oligodeoxynucleotides in mouse bone marrow-derived mesenchymal stem cells. Gene Ther 15:1035-48
Pattanayak, Vikram; Gifford, Lida K; Lu, Ponzy et al. (2008) Observed versus predicted structure of fluorescent self-quenching reporter molecules (SQRM): caveats with respect to the use of ""stem-loop"" oligonucleotides as probes for mRNA folding. RNA 14:657-65
Tang, XinJing; Swaminathan, Jyothishmathi; Gewirtz, Alan M et al. (2008) Regulating gene expression in human leukemia cells using light-activated oligodeoxynucleotides. Nucleic Acids Res 36:559-69
Silva, Ana Luisa; Ribeiro, Patricia; Inacio, Angela et al. (2008) Proximity of the poly(A)-binding protein to a premature termination codon inhibits mammalian nonsense-mediated mRNA decay. RNA 14:563-76

Showing the most recent 10 out of 45 publications