The continuing goal of the Flow Cytometry Core is to provide acquisition and analysis of any flow cytometry data needed by program investigators. For evaluation of plasma, serum, and cell culture supernatants, the core provides staining, acquisition, and analysis of expression of as many as 30 cytokines / chemokines / cytotoxic proteins / phospho-proteins per sample using the Cytometric Bead Array (CBA) multiplexing platform. The core has been providing 8-color immunophenotyping and is preparing to extend this to 10-color detection. The core stains, acquires, and analyzes the levels of expression of intracellular proteins (cytokines such as IL-6, IL-10, IL-12) and phospho-proteins (phospho-Stat3). The Aldefluor assay is employed by the core to examine expression of aldehyde dehydrogenase, which is reportedly expressed at increased levels in cancer stem cells. The core has previously demonstrated the ability of its cell sorter to perform high-purity sorting of gene-transduced cells co-expressing green fluorescent protein (GFP).
This core provides significant and essential resources to the four projects in the PPG as it allows identification of plasma/serum and supernatant analytes, cell surface immunophenotypes, quantification of intracellular proteins and phospho-proteins, reactive oxygen species (ROS), and apoptosis. The significance of work done for Projects 1-4 can be found in reports of those projects.
|DuBois, Steven G; Marachelian, Araz; Fox, Elizabeth et al. (2016) Phase I Study of the Aurora A Kinase Inhibitor Alisertib in Combination With Irinotecan and Temozolomide for Patients With Relapsed or Refractory Neuroblastoma: A NANT (New Approaches to Neuroblastoma Therapy) Trial. J Clin Oncol 34:1368-75|
|Trieu, Megan; DuBois, Steven G; Pon, Elizabeth et al. (2016) Impact of Whole-Body Radiation Dose on Response and Toxicity in Patients With Neuroblastoma After Therapy With 131 I-Metaiodobenzylguanidine (MIBG). Pediatr Blood Cancer 63:436-42|
|DuBois, Steven G; Groshen, Susan; Park, Julie R et al. (2015) Phase I Study of Vorinostat as a Radiation Sensitizer with 131I-Metaiodobenzylguanidine (131I-MIBG) for Patients with Relapsed or Refractory Neuroblastoma. Clin Cancer Res 21:2715-21|
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|Yanik, Gregory A; Villablanca, Judith G; Maris, John M et al. (2015) 131I-metaiodobenzylguanidine with intensive chemotherapy and autologous stem cell transplantation for high-risk neuroblastoma. A new approaches to neuroblastoma therapy (NANT) phase II study. Biol Blood Marrow Transplant 21:673-81|
|HaDuong, Josephine H; Blavier, Laurence; Baniwal, Sanjeev K et al. (2015) Interaction between bone marrow stromal cells and neuroblastoma cells leads to a VEGFA-mediated osteoblastogenesis. Int J Cancer 137:797-809|
|Wang, L L; Teshiba, R; Ikegaki, N et al. (2015) Augmented expression of MYC and/or MYCN protein defines highly aggressive MYC-driven neuroblastoma: a Children's Oncology Group study. Br J Cancer 113:57-63|
|DuBois, S G; Allen, S; Bent, M et al. (2015) Phase I/II study of (131)I-MIBG with vincristine and 5 days of irinotecan for advanced neuroblastoma. Br J Cancer 112:644-9|
|Cage, Tene Aneka; Chanthery, Yvan; Chesler, Louis et al. (2015) Downregulation of MYCN through PI3K Inhibition in Mouse Models of Pediatric Neural Cancer. Front Oncol 5:111|
|Huang, Shih-ying; Bolch, Wesley E; Lee, Choonsik et al. (2015) Patient-specific dosimetry using pretherapy [Â¹Â²â´I]m-iodobenzylguanidine ([Â¹Â²â´I]mIBG) dynamic PET/CT imaging before [Â¹Â³Â¹I]mIBG targeted radionuclide therapy for neuroblastoma. Mol Imaging Biol 17:284-94|
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