The Microscopy Service and Equipment Core continues to support each ofthe projects with electron microscopy, confocal microscopy, and digital fluorescence microscopy equipment, services, and support. Users are trained in confocal and fluorescence microscope use, sample preparation, and image analysis. Ultrastructural analysis of cells and tissues is performed by traditional embedded section and embedment-free transmission electron microscopy, and by scanning electron microscopy. High resolution electron microscopic immuno-localization of proteins with colloidal gold coupled second antibodies is performed on embedded and resinless sections. Proteins and nucleic acids are localized in three dimensions by confocal microscopy, or alternatively image stack deconvolution. Live cell microscopy is performed to achieve the biochemical characterization of macromolecular assembly;PRAP measures binding kinetics while FRET can establish complex composition. The capability for long-term time lapse experiments is offered. The Microscopy Core offers hardware, software, and training for image processing and analysis.

Public Health Relevance

Imaging technologies, and especially microscopy, are in a period of explosive growth in sophistication, resolution, and power. Increasing, advanced microscopy techniques are essential tools for understanding the changes in cell and tissue organization which are central to our emerging understanding of cancer.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Program Projects (P01)
Project #
5P01CA082834-14
Application #
8601048
Study Section
Special Emphasis Panel (ZCA1-RPRB-0)
Project Start
Project End
Budget Start
2013-02-01
Budget End
2014-01-31
Support Year
14
Fiscal Year
2013
Total Cost
$182,961
Indirect Cost
$51,294
Name
University of Vermont & St Agric College
Department
Type
DUNS #
066811191
City
Burlington
State
VT
Country
United States
Zip Code
05405
Barutcu, A Rasim; Hong, Deli; Lajoie, Bryan R et al. (2016) RUNX1 contributes to higher-order chromatin organization and gene regulation in breast cancer cells. Biochim Biophys Acta 1859:1389-1397
Varela, Nelson; Aranguiz, Alejandra; Lizama, Carlos et al. (2016) Mitotic Inheritance of mRNA Facilitates Translational Activation of the Osteogenic-Lineage Commitment Factor Runx2 in Progeny of Osteoblastic Cells. J Cell Physiol 231:1001-14
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Barutcu, A Rasim; Lajoie, Bryan R; Fritz, Andrew J et al. (2016) SMARCA4 regulates gene expression and higher-order chromatin structure in proliferating mammary epithelial cells. Genome Res 26:1188-201
VanOudenhove, Jennifer J; Medina, Ricardo; Ghule, Prachi N et al. (2016) Transient RUNX1 Expression during Early Mesendodermal Differentiation of hESCs Promotes Epithelial to Mesenchymal Transition through TGFB2 Signaling. Stem Cell Reports 7:884-896
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Taipaleenmäki, Hanna; Farina, Nicholas H; van Wijnen, Andre J et al. (2016) Antagonizing miR-218-5p attenuates Wnt signaling and reduces metastatic bone disease of triple negative breast cancer cells. Oncotarget :
VanOudenhove, Jennifer J; Grandy, Rodrigo A; Ghule, Prachi N et al. (2016) Unique Regulatory Mechanisms for the Human Embryonic Stem Cell Cycle. J Cell Physiol :
Barutcu, A Rasim; Fritz, Andrew J; Zaidi, Sayyed K et al. (2016) C-ing the Genome: A Compendium of Chromosome Conformation Capture Methods to Study Higher-Order Chromatin Organization. J Cell Physiol 231:31-5
Wu, Qiong; Sharma, Soni; Cui, Hang et al. (2016) Targeting the chromatin remodeling enzyme BRG1 increases the efficacy of chemotherapy drugs in breast cancer cells. Oncotarget 7:27158-75

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