The Microscopy Service and Equipment Core continues to support each ofthe projects with electron microscopy, confocal microscopy, and digital fluorescence microscopy equipment, services, and support. Users are trained in confocal and fluorescence microscope use, sample preparation, and image analysis. Ultrastructural analysis of cells and tissues is performed by traditional embedded section and embedment- free transmission electron microscopy, and by scanning electron microscopy. High resolution electron microscopic immuno-localization of proteins with colloidal gold coupled second antibodies is performed on embedded and resinless sections. Proteins and nucleic acids are localized in three dimensions by confocal microscopy, or alternatively image stack deconvolution. Live cell microscopy is performed to achieve the biochemical characterization of macromolecular assembly;PRAP measures binding kinetics while FRET can establish complex composition. The capability for long-term time lapse experiments is offered. The Microscopy Core offers hardware, software, and training for image processing and analysis.
Imaging technologies, and especially microscopy, are in a period of explosive growth in sophistication, resolution, and power. Increasing, advanced microscopy techniques are essential tools for understanding the changes in cell and tissue organization which are central to our emerging understanding of cancer.
|Zhang, Xuhui; Akech, Jacqueline; Browne, Gillian et al. (2015) Runx2-Smad signaling impacts the progression of tumor-induced bone disease. Int J Cancer 136:1321-32|
|Kapinas, Kristina; Kim, Heesun; Mandeville, Matthew et al. (2015) microRNA-mediated survivin control of pluripotency. J Cell Physiol 230:63-70|
|Gordon, Jonathan A R; Montecino, Martin A; Aqeilan, Rami I et al. (2014) Epigenetic pathways regulating bone homeostasis: potential targeting for intervention of skeletal disorders. Curr Osteoporos Rep 12:496-506|
|Barutcu, A Rasim; Tai, Phillip W L; Wu, Hai et al. (2014) The bone-specific Runx2-P1 promoter displays conserved three-dimensional chromatin structure with the syntenic Supt3h promoter. Nucleic Acids Res 42:10360-72|
|Stumpff, Jason; Ghule, Prachi N; Shimamura, Akiko et al. (2014) Spindle microtubule dysfunction and cancer predisposition. J Cell Physiol 229:1881-3|
|Zaidi, Sayyed K; Grandy, Rodrigo A; Lopez-Camacho, Cesar et al. (2014) Bookmarking target genes in mitosis: a shared epigenetic trait of phenotypic transcription factors and oncogenes? Cancer Res 74:420-5|
|Dutta, Anindita; Li, Jing; Lu, Huimin et al. (2014) Integrin ?v?6 promotes an osteolytic program in cancer cells by upregulating MMP2. Cancer Res 74:1598-608|
|Tai, Phillip W L; Zaidi, Sayyed K; Wu, Hai et al. (2014) The dynamic architectural and epigenetic nuclear landscape: developing the genomic almanac of biology and disease. J Cell Physiol 229:711-27|
|Tai, Phillip W L; Wu, Hai; Gordon, Jonathan A R et al. (2014) Epigenetic landscape during osteoblastogenesis defines a differentiation-dependent Runx2 promoter region. Gene 550:1-9|
|Lamba, Gurpreet; Zaidi, Sayyed Kaleem; Luebbers, Kimberly et al. (2014) Epigenetic landscape of acute myelogenous leukemia--moving toward personalized medicine. J Cell Biochem 115:1669-72|
Showing the most recent 10 out of 132 publications