The Microscopy Service and Equipment Core continues to support each ofthe projects with electron microscopy, confocal microscopy, and digital fluorescence microscopy equipment, services, and support. Users are trained in confocal and fluorescence microscope use, sample preparation, and image analysis. Ultrastructural analysis of cells and tissues is performed by traditional embedded section and embedment- free transmission electron microscopy, and by scanning electron microscopy. High resolution electron microscopic immuno-localization of proteins with colloidal gold coupled second antibodies is performed on embedded and resinless sections. Proteins and nucleic acids are localized in three dimensions by confocal microscopy, or alternatively image stack deconvolution. Live cell microscopy is performed to achieve the biochemical characterization of macromolecular assembly;PRAP measures binding kinetics while FRET can establish complex composition. The capability for long-term time lapse experiments is offered. The Microscopy Core offers hardware, software, and training for image processing and analysis.

Public Health Relevance

Imaging technologies, and especially microscopy, are in a period of explosive growth in sophistication, resolution, and power. Increasing, advanced microscopy techniques are essential tools for understanding the changes in cell and tissue organization which are central to our emerging understanding of cancer.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Program Projects (P01)
Project #
5P01CA082834-15
Application #
8444561
Study Section
Special Emphasis Panel (ZCA1-RPRB-0)
Project Start
Project End
Budget Start
2014-02-01
Budget End
2015-01-31
Support Year
15
Fiscal Year
2014
Total Cost
$230,127
Indirect Cost
$49,111
Name
University of Vermont State Agr Coll
Department
Type
DUNS #
City
Burlington
State
VT
Country
United States
Zip Code
Araya, H├ęctor F; Sepulveda, Hugo; Lizama, Carlos O et al. (2018) Expression of the ectodomain-releasing protease ADAM17 is directly regulated by the osteosarcoma and bone-related transcription factor RUNX2. J Cell Biochem 119:8204-8219
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