The aims of the Animal/Pathology Core are to:
AIM A. Collect, process, archive and distribute human and animal tissues (Projects 1,2,3,4) AIM B. Perform tumor implantations and illuminations;assist in other treatments of mice (Projects 2,3,4) AIM C. Assist in mouse autopsies (Dr. Durham) and coordinate the movement of animal and human tumor samples for immunohistochemical analysis between Pathology (human or veterinary, as relevant) and the laboratories of Project Leaders. The strength of Core B is that it will provide consistent animal models, animal treatments, tissue collection and pathologic analyses for the projects in this PPG. Given the high tech nature of delivering PDT, this is particularly important. The Core will act as a center point to assure that tissues will be collected and distributed in a consistent manner, based on our SOPS, and this process will be well documented using detailed data collection forms included in the Appendix of Core B. Dr. Sydney Evans will be the director of Core B;Dr. Durham will be responsible for the animal-related immunohistochemistry tasks after autopsy (Aim C). The functions of Core B can be separated into the ANIMAL and the PATHOLOGY subcores. The ANIMAL SUBCORE will be responsible for 1) Aiding the PIs in developing, submitting and maintaining IACUC proposals 2) Ordering animals 3) Performing inter-project animal work, including tumor implantation, illumination, tissue collection The PATHOLOGY SUBCORE will: 1) Aid in the collection, processing, and archiving or storage of animal and human tissues/blood 2) Oversee murine autopsies, collection and submission of tissues for staining/histopathological review

Public Health Relevance

Core B The relevance of this core lies in its centralization of critical experimental aspects of tissue collection and murine treatment for the PPG to a single core, with oversight by a single highly-trained Investigator. Results gained thorugh use of the resources provided by this core will be generalizable to a wide range of PDT applications that are performed in the context of surgery.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Program Projects (P01)
Project #
2P01CA087971-11A1
Application #
8741239
Study Section
Special Emphasis Panel (ZCA1-RPRB-B (M1))
Project Start
2000-07-01
Project End
2015-06-30
Budget Start
2014-09-10
Budget End
2015-06-30
Support Year
11
Fiscal Year
2014
Total Cost
$272,214
Indirect Cost
$102,080
Name
University of Pennsylvania
Department
Type
DUNS #
042250712
City
Philadelphia
State
PA
Country
United States
Zip Code
19104
Kim, Michele M; Darafsheh, Arash; Ahmad, Mahmoud et al. (2016) PDT Dose Dosimeter for Pleural Photodynamic Therapy. Proc SPIE Int Soc Opt Eng 9694:96940Y
Sterman, Daniel H; Alley, Evan; Stevenson, James P et al. (2016) Pilot and Feasibility Trial Evaluating Immuno-Gene Therapy of Malignant Mesothelioma Using Intrapleural Delivery of Adenovirus-IFNα Combined with Chemotherapy. Clin Cancer Res 22:3791-800
Ong, Yi Hong; Zhu, Timothy C (2016) Analytic function for predicting light fluence rate of circular fields on a semi-infinite turbid medium. Opt Express 24:26261-26281
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Grossman, Craig E; Carter, Shirron L; Czupryna, Julie et al. (2016) Fluence Rate Differences in Photodynamic Therapy Efficacy and Activation of Epidermal Growth Factor Receptor after Treatment of the Tumor-Involved Murine Thoracic Cavity. Int J Mol Sci 17:
Penjweini, Rozhin; Kim, Michele M; Dimofte, Andrea et al. (2016) Deformable medical image registration of pleural cavity for photodynamic therapy by using finite-element based method. Proc SPIE Int Soc Opt Eng 9701:970106
Zhu, Timothy C; Lu, Amy; Ong, Yi-Hong (2016) An improved analytic function for predicting light fluence rate in circular fields on a semi-infinite geometry. Proc SPIE Int Soc Opt Eng 9706:97061D
Penjweini, Rozhin; Kim, Michele M; Liu, Baochang et al. (2016) Evaluation of the 2-(1-Hexyloxyethyl)-2-devinyl pyropheophorbide (HPPH) mediated photodynamic therapy by macroscopic singlet oxygen modeling. J Biophotonics 9:1344-1354
Penjweini, Rozhin; Kim, Michele M; Finlay, Jarod C et al. (2016) Investigating the impact of oxygen concentration and blood flow variation on photodynamic therapy. Proc SPIE Int Soc Opt Eng 9694:
Gemmell, Nathan R; McCarthy, Aongus; Kim, Michele M et al. (2016) A compact fiber-optic probe-based singlet oxygen luminescence detection system. J Biophotonics :

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