The Animal Core will continue to provide investigators of each project with assistance in the conduct of research involving animals. The core will provide a focus of expertise that is germane to all projects and also efficiently utilize valuable resources. The core's PI, Dr. E. Keller has extensive experience with prostate cancer xenograft development and rodent models of prostate cancer including intratibial and intracardiac injection models. Dr. Keller will oversee the scientific direction of the core. The core's day-to-day activities will be overseen by Dr. Jill Keller, an American College of Laboratory Animal Medicine certified veterinarian. The Animal Core activities include: - Creating animal models to be used for the projects. For example, implantation of vertebrae of mice to create vossicle models will be performed by the Animal Core. - Assisting with animal experiments. Core personnel will coordinate and assist or instruct in the performance of standardized procedures such as intratibial and intracardiac injection, anesthesia, etc. Providing imaging capability for animal studies. The core will perform faxitron radiographs, bone densitometry (Dexa or pQCT), and bioluminescence. - Maintaining breeding colonies for various specialized mice as required for various projects. Specific genetically-modified mice will be maintained as required. - Development of new animal models of bone metastasis. Dr. J. Keller will test xenografts and cell lines derived from the Prostate SPORE for their ability to serve as models of skeletal metastasis.

Public Health Relevance

Bone metastasis are a serious and frequent clinical complication of prostate cancer. This core will provide animal models to help identify mechanisms and targets to attack this important aspect of prostate cancer.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Program Projects (P01)
Project #
5P01CA093900-10
Application #
8464649
Study Section
Special Emphasis Panel (ZCA1-RPRB-O)
Project Start
2013-05-01
Project End
2014-04-30
Budget Start
2013-05-01
Budget End
2014-04-30
Support Year
10
Fiscal Year
2013
Total Cost
$319,394
Indirect Cost
$71,028
Name
University of Michigan Ann Arbor
Department
Type
DUNS #
073133571
City
Ann Arbor
State
MI
Country
United States
Zip Code
48109
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Yumoto, Kenji; Eber, Matthew R; Wang, Jingcheng et al. (2016) Axl is required for TGF-β2-induced dormancy of prostate cancer cells in the bone marrow. Sci Rep 6:36520
Cackowski, Frank C; Eber, Matthew R; Rhee, James et al. (2016) Mer Tyrosine Kinase Regulates Disseminated Prostate Cancer Cellular Dormancy. J Cell Biochem :
Chen, F; Dai, Z; Kang, Y et al. (2016) Effects of zoledronic acid on bone fusion in osteoporotic patients after lumbar fusion. Osteoporos Int 27:1469-76
Amend, Sarah R; Roy, Sounak; Brown, Joel S et al. (2016) Ecological paradigms to understand the dynamics of metastasis. Cancer Lett 380:237-42
van der Toom, Emma E; Verdone, James E; Pienta, Kenneth J (2016) Disseminated tumor cells and dormancy in prostate cancer metastasis. Curr Opin Biotechnol 40:9-15
Amend, Sarah R; Valkenburg, Kenneth C; Pienta, Kenneth J (2016) Murine Hind Limb Long Bone Dissection and Bone Marrow Isolation. J Vis Exp :
Lee, Eunsohl; Wang, Jingcheng; Yumoto, Kenji et al. (2016) DNMT1 Regulates Epithelial-Mesenchymal Transition and Cancer Stem Cells, Which Promotes Prostate Cancer Metastasis. Neoplasia 18:553-66

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