CORE B ABSTRACT Core B, the Molecular Pathology and Imaging Core (MPIC;formerly the Morphology Core), was established in 1995 and has been a part of this Program Project since its inception. During this time, MPIC has served as a model core facility for numerous other program projects and centers at the University of Pennsylvania and throughout the country. The overarching goal of MPIC is to provide exceptional service and support to the P01 investigators and their laboratories, accomplished through two Specific Aims: 1) To provide essential, cost- effective, and high quality services and support to the esophageal cancer P01 investigators and their laboratories for the preparation, sectioning, staining, and interpretation of tissue sections, both animal and human, as well as cultured cells;and 2) To assist in planning and optimizing (a) in situ hybridization (ISH), immunohistochemistry (IHC), and immunofluorescence (IF) experiments and (b) microscopy and image analyses for the esophageal cancer P01 members and their laboratories and to provide training in these techniques, as well as perform these services as requested. MPIC is lead by an experienced Director (Dr. Jonathan Katz), Technical Director (Mr. Adam Bedenbaugh), and Consultant (Dr. Andres Klein-Szanto). Moreover, MPIC is a unique entity at the University of Pennsylvania and the Dana Farber Cancer Institute and possesses specific expertise in processing and analyses of all tissue types relevant to this P01. MPIC continues to provide essential, routine services, such as tissue processing and staining, as well as advanced technologies, such as spinning-disk confocal microscopy with an environmental chamber for live-cell imaging, to P01 investigators and their lab personnel. MPIC also fosters cost-effective approaches and promotes new and exciting directions for research. In addition, MPIC offers special benefits to members of this P01 that are not available to other users including esophageal cancer specific TMAs, significant subsidies for services, priority services, and access to Dr. Klein-Szanto, an expert clinical pathologist. Since the last submission of this application, we have continued to incorporate and integrate new services and technologies and, reflective of these changes and the services currently provided to our users, have changed the name of the Core from the Morphology Core to the Molecular Pathology and Imaging Core. Recent enhancements include relocation into more than 1000 square feet of newly renovated space, upgrade of a Yokagawa CSU-10 spinning disk confocal microscope for live-cell imaging, integration of an Olympus IX81 live-cell imaging system, and incorporation of on-line scheduling and ordering. Thus, MPIC provides exceptional utility, quality, value, experience, service, and commitment to this P01, and this P01 receives top priority.

Public Health Relevance

Core B, the Molecular Pathology and Imaging Core (MPIC;formerly the Morphology Core), provides a number of key services for P01 investigators and their laboratories including the processing and staining of tissues relevant to this P01, microscopy, and image analysis. Notably, MPIC offers special benefits to members of this P01 that are not available to other users. Overall, MPIC provides exceptional utility, quality, value, experience, service, and commitment to this P01, and this P01 receives top priority.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Program Projects (P01)
Project #
2P01CA098101-11
Application #
8741113
Study Section
Special Emphasis Panel (ZCA1-RPRB-O (M1))
Project Start
2002-12-01
Project End
2019-06-30
Budget Start
2014-08-01
Budget End
2015-06-30
Support Year
11
Fiscal Year
2014
Total Cost
$146,145
Indirect Cost
$41,319
Name
University of Pennsylvania
Department
Type
DUNS #
042250712
City
Philadelphia
State
PA
Country
United States
Zip Code
19104
Tetreault, Marie-Pier; Weinblatt, Daniel; Shaverdashvili, Khvaramze et al. (2016) KLF4 transcriptionally activates non-canonical WNT5A to control epithelial stratification. Sci Rep 6:26130
Yoshida, Akihiro; Lee, Eric K; Diehl, J Alan (2016) Induction of Therapeutic Senescence in Vemurafenib-Resistant Melanoma by Extended Inhibition of CDK4/6. Cancer Res 76:2990-3002
Kong, Jianping; Whelan, Kelly A; Laczkó, Dorottya et al. (2016) Autophagy levels are elevated in barrett's esophagus and promote cell survival from acid and oxidative stress. Mol Carcinog 55:1526-1541
Dotto, G Paolo; Rustgi, Anil K (2016) Squamous Cell Cancers: A Unified Perspective on Biology and Genetics. Cancer Cell 29:622-37
Qie, Shuo; Diehl, J Alan (2016) Cyclin D1, cancer progression, and opportunities in cancer treatment. J Mol Med (Berl) 94:1313-1326
Tétreault, Marie-Pier; Weinblatt, Daniel; Ciolino, Jody Dyan et al. (2016) Esophageal Expression of Active IκB Kinase-β in Mice Up-Regulates Tumor Necrosis Factor and Granulocyte-Macrophage Colony-Stimulating Factor, Promoting Inflammation and Angiogenesis. Gastroenterology 150:1609-1619.e11
Whelan, Kelly A; Merves, Jamie F; Giroux, Veronique et al. (2016) Autophagy mediates epithelial cytoprotection in eosinophilic oesophagitis. Gut :
Lin, E W; Karakasheva, T A; Hicks, P D et al. (2016) The tumor microenvironment in esophageal cancer. Oncogene 35:5337-5349
Shearin, Abigail L; Monks, Bobby R; Seale, Patrick et al. (2016) Lack of AKT in adipocytes causes severe lipodystrophy. Mol Metab 5:472-9
Facompre, Nicole D; Harmeyer, Kayla M; Sole, Xavier et al. (2016) JARID1B Enables Transit between Distinct States of the Stem-like Cell Population in Oral Cancers. Cancer Res 76:5538-49

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