CORE C ABSTRACT The Molecular Biology/Gene Expression Core Facility will provide critical services to support the scientific goals of this Program Project: 1) Molecular Reagent Bank- contains specific plasmids, expression constructs, reporter constructs, antibodies, RNA samples, and retroviral/adenoviral/lentiviral vectors of relevance to the study of esophageal carcinogenesis. As a result, these reagents have been organized and centralized. In addition, we will continue to store newly available reagents;2) Image Analysis and Gene Expression Quantification-multiple instruments are available, such as (but not limited to) Phosphorimaging, Gel and Chemidoc, Agilent Bioanalyzer and Odyssey Infrared Protein Imaging System, real-time quantitative PCR, and Luminometers for gene transcription analysis, a nanodrop, and flow cytometer;3) Genomic Services (subsidized) with gene microarrays (Affymetrix platform) through the Penn microarray facility, ultra-high throughput DNA sequencing for RNA- and CHIP-seq, and bioinformatics support;and 4) Esophageal Cell Line Bank (murine and human) with primary, immortalized, transformed and newly genetically engineered esophageal cell lines for use by the Projects. The Molecular Biology/Gene Expression Core provides quality assurance, quality control, cost-effectiveness, timeliness and efficiency in its services. This Core works closely with all the other Cores, especially the Molecular Pathology and Imaging Core (MPIC) to avoid duplication and furnish coordinated approaches for the Projects. The Projects benefit tremendously from this Core in advancing their interrelated hypotheses and Specific Aims. In addition, this Core is dynamic in responding to evolving P01 needs, anticipating future P01 directions, and integrating emerging technologies.
The Molecular Biology/Gene Expression Core (MBC) provides a number of high technology services essential for the experiments focused on esophageal cancer that have been proposed by all three of the P01 investigators. These include access to expensive core equipment required for the analysis of protein, RNA, and DNA obtained from esophageal tissues and cell lines, ultra-high throughput DNA sequencing and bioinformatic analyses, as well as a repository of molecular reagents and esophageal cell lines. Interaction between the MBC and the other cores on this P01 provides the investigators with a dynamic and responsive environment the facilitates research into the biology of esophageal cancer.
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