The Pathology and Laboratory Support Core is an effective nucleus that supports the various studies of the program project by providing routine and advanced technical preparations, including morphological analysis of cells, tissue and organ preparations. The core provides diagnostic light microscopic evaluation, epifluorescent microscopy of histological sections and immunohistochemical interpretation of human and murine prostatic tissue and prostate cancer bone metastases. In addition, technologically advanced expert assistance in a broad range of methods, including quantitative RT-PCR and tissue in-situ hybridization are available. Program project investigators utilizing animal necropsies also have access to sophisticated techniques, such as transmission electron microscopy and image analysis/morphometry. Facilities available include histology (special stains, frozen, paraffin- and plastic-embedded sections) and immunohistochemistry. A key advantage of the core is that it enables unified characterization and dissemination of a shared set of histologic standards. Key to the study of the tumor-bone-stromal interaction is the ability to isolate relatively pure samples of the tumor and the surrounding stroma. Particularly relevant to our program project is the ability to do so in the primary tumor as well as in bone specimens with metastasis.The pathology core will a) procure fresh prostatic tissue, including: primary tumor, tumor-related stroma, benign epithelium, metastatic bone specimens, and stroma unrelated to tumor from clinical specimens under direct morphologic visualization using laser capture microscopy for molecular analyses;b) serve as the morphology core, providing prostate cancer diagnostic expertise for both clinical specimens and animal models of prostate cancer, maintain frozen section and light microscopic facilities, histochemistry, immunohistochemistry and in situ hybridization microscopic support;and c) provide ample well-characterized, archived pathologic samples for screening of candidate molecules of potential mechanistic importance, validation of cell culture and animal model experimental data anddiscovery of novel biomarkers, including high throughput analysis - tissue microarray.

Public Health Relevance

Core C will continue to provide both diagnostic and molecular analysis of cell and tissue samples from human and animals. This will allow for more comprehensive quality testing with wider variety of advanced methods. Procedures are standardized and quality-controlled, leading to greater technical uniformity and allowing for direct comparisons between projects. Investigators will continue to have ready access to sophisticated techniques such as transmission electron microscopy and image analysis.

National Institute of Health (NIH)
National Cancer Institute (NCI)
Research Program Projects (P01)
Project #
Application #
Study Section
Special Emphasis Panel (ZCA1-GRB-S)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Cedars-Sinai Medical Center
Los Angeles
United States
Zip Code
Smith, Bethany N; Bhowmick, Neil A (2016) Role of EMT in Metastasis and Therapy Resistance. J Clin Med 5:
Torres, Mylin A; Yang, Xiaofeng; Noreen, Samantha et al. (2016) The Impact of Axillary Lymph Node Surgery on Breast Skin Thickening During and After Radiation Therapy for Breast Cancer. Int J Radiat Oncol Biol Phys 95:590-6
Farach, Andrew; Ding, Yi; Lee, MinJae et al. (2016) Neuronal Trans-Differentiation in Prostate Cancer Cells. Prostate 76:1312-25
Masko, Elizabeth M; Alfaqih, Mahmoud A; Solomon, Keith R et al. (2016) Evidence for Feedback Regulation Following Cholesterol Lowering Therapy in a Prostate Cancer Xenograft Model. Prostate :
Li, Qinlong; Yin, Lijuan; Jones, Lawrence W et al. (2016) Keratin 13 expression reprograms bone and brain metastases of human prostate cancer cells. Oncotarget :
Miyahira, Andrea K; Lang, Joshua M; Den, Robert B et al. (2016) Multidisciplinary intervention of early, lethal metastatic prostate cancer: Report from the 2015 Coffey-Holden Prostate Cancer Academy Meeting. Prostate 76:125-39
Grigore, Alexandru Dan; Jolly, Mohit Kumar; Jia, Dongya et al. (2016) Tumor Budding: The Name is EMT. Partial EMT. J Clin Med 5:
Fong, Eliza L S; Wan, Xinhai; Yang, Jun et al. (2016) A 3D in vitro model of patient-derived prostate cancer xenograft for controlled interrogation of in vivo tumor-stromal interactions. Biomaterials 77:164-72
Liu, Sandy; Cadaneanu, Radu M; Zhang, Baohui et al. (2016) Keratin 13 Is Enriched in Prostate Tubule-Initiating Cells and May Identify Primary Prostate Tumors that Metastasize to the Bone. PLoS One 11:e0163232
You, Sungyong; Knudsen, Beatrice S; Erho, Nicholas et al. (2016) Integrated Classification of Prostate Cancer Reveals a Novel Luminal Subtype with Poor Outcome. Cancer Res 76:4948-58

Showing the most recent 10 out of 190 publications