The Molecular Diagnostic and Characterization Core (MDCC) will serve as a critical component of the """"""""Host Defense Regulation and Viral Oncogenesis"""""""" Program, providing services and expertise in purification, detection, quantitation and characterization of nucleic acid sequences using real-time RT-PCR applications, DNA sequencing and DNA/RNA array analysis. The MDCC is equipped with the latest in molecular analysis tools and expertise to provide the following services to support the research aims outlined for each project in this proposal: 1: Perform quantitative gene expression analysis for viral and cellular genes. 2: Perform DNA sequencing on genes or gene products from viral and cellular specimens. 3: Support development and analysis of research and clinical specimens using DNA/RNA arrays. 4: Perform standardized production and quantitation of plasmid DNA for transfection experiments using cell lines. Perform standardized RNA and DNA extraction and purification from research specimens including cell lines and primary tumor samples. 6: Provide professional expertise in the development of new diagnostic and detection tools, evaluation of experimental results, and development of subsequent experimental directions. The main support function of the MDCC is to provide expertise and access to key molecular analysis platforms for the detection and quantitation of target gene products in tumor cells as well as develop critical tools for evaluating altered gene expression and probing new observations gained from the proposed projects. The MDCC will design, develop and optimize assays for each of the projects as they progress and pursue future directions. These validated tools will be shared with the Tissue/Pathology Core for primary tumor characterization where warranted.

Public Health Relevance

Through support of the program projects described in this proposal, the MDCC will aid in the identification of new biomarkers in viral-mediated cancers that may lead to the development of new diagnostic and prognostic tools. These tools would subsequently be translated for clinical applications in the early diagnosis and management of patients with viral associated cancers. The benefit to overall public health is to provide new assays and technologies to best identify and manage the treatment of patients with these forms of cancer.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Program Projects (P01)
Project #
5P01CA128115-05
Application #
8546190
Study Section
Special Emphasis Panel (ZCA1-GRB-S)
Project Start
Project End
Budget Start
2013-08-01
Budget End
2014-07-31
Support Year
5
Fiscal Year
2013
Total Cost
$113,036
Indirect Cost
$37,027
Name
University of Miami School of Medicine
Department
Type
DUNS #
052780918
City
Coral Gables
State
FL
Country
United States
Zip Code
33146
Betancourt, Dillon; Ramos, Juan Carlos; Barber, Glen N (2015) Retargeting Oncolytic Vesicular Stomatitis Virus to Human T-Cell Lymphotropic Virus Type 1-Associated Adult T-Cell Leukemia. J Virol 89:11786-800
Zhou, Qinjie; Lavorgna, Alfonso; Bowman, Melissa et al. (2015) Aryl Hydrocarbon Receptor Interacting Protein Targets IRF7 to Suppress Antiviral Signaling and the Induction of Type I Interferon. J Biol Chem 290:14729-39
Bayraktar, Ulas Darda; Diaz, Luis A; Ashlock, Brittany et al. (2014) Zidovudine-based lytic-inducing chemotherapy for Epstein-Barr virus-related lymphomas. Leuk Lymphoma 55:786-94
Gao, Linlin; Harhaj, Edward William (2013) HSP90 protects the human T-cell leukemia virus type 1 (HTLV-1) tax oncoprotein from proteasomal degradation to support NF-κB activation and HTLV-1 replication. J Virol 87:13640-54
Charoenthongtrakul, Soratree; Gao, Linlin; Parvatiyar, Kislay et al. (2013) RING finger protein 11 targets TBK1/IKKi kinases to inhibit antiviral signaling. PLoS One 8:e53717
Bhatt, Shruti; Ashlock, Brittany M; Toomey, Ngoc L et al. (2013) Efficacious proteasome/HDAC inhibitor combination therapy for primary effusion lymphoma. J Clin Invest 123:2616-28
Lavorgna, Alfonso; Harhaj, Edward W (2013) Is there a role for ubiquitin or SUMO in human T-cell leukemia virus type 2 Tax-induced NF-*B activation? Future Virol 8:223-227
Shembade, Noula; Harhaj, Edward W (2012) Regulation of NF-κB signaling by the A20 deubiquitinase. Cell Mol Immunol 9:123-30
Lavorgna, Alfonso; Harhaj, Edward W (2012) EBV LMP1: New and shared pathways to NF-ýýB activation. Proc Natl Acad Sci U S A 109:2188-9
Harhaj, Edward W; Dixit, Vishva M (2012) Regulation of NF-κB by deubiquitinases. Immunol Rev 246:107-24

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