While 80% of melanomas harbor mutation in genes that activate the MEK-MAPK signaling pathway, over 50% of melanomas also exhibit upregulation of the AKT/PI3K signaling cascade. Given that inhibitors to components of the MEK/MAPK signaling cascade are not sufficient enough to halt melanoma progression, it is now recognized that combination therapy in melanoma is inevitable. More and more studies provide sound support for the notion that PI3K/Akt is likely to serve as the excellent candidate for combined therapy. Among mechanisms underlying PI3K/Akt contribution to melanoma development is its effect on hypoxia and tumor microenvironment, although underlying mechanisms are not well understood. Among Pten/Akt effectors that play important role in the regulation of HIFIa availability is the ubiquitin ligase Siah2, which is transcriptionally upregulated by Akt. Correspondingly, increased expression of Siah2 is found in malignant melanoma. Further, inhibition of Siah2 effectively blocks melanoma growth and progression. These finding provide the rationale to develop inhibitors to Akt and Siah2 as select targets for treatment of this tumor type. Thus, we propose to use a combination of chemical library screening, structure-based design and medicinal chemistry approaches to obtain potent and selective inhibitors of Akt and Siah. In close collaboration with Project 1 and Core B and C the compounds will be tested in vitro, in 3D cell cultures and in in vivo efficacy and toxicity studies. Overall, our main objective is to develop novel safe and effective agents that by targeting specific genes such as Akt and Siah, which would provide compelling alternative strategies for the treatment of melanoma.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Program Projects (P01)
Project #
5P01CA128814-05
Application #
8528359
Study Section
Special Emphasis Panel (ZCA1-RPRB-O)
Project Start
Project End
Budget Start
2013-07-01
Budget End
2014-06-30
Support Year
5
Fiscal Year
2013
Total Cost
$529,342
Indirect Cost
$257,885
Name
Sanford-Burnham Medical Research Institute
Department
Type
DUNS #
020520466
City
La Jolla
State
CA
Country
United States
Zip Code
92037
García-Jiménez, Custodia; Goding, Colin R (2018) Starvation and Pseudo-Starvation as Drivers of Cancer Metastasis through Translation Reprogramming. Cell Metab :
Theodosakis, Nicholas; Langdon, Casey G; Micevic, Goran et al. (2018) Inhibition of isoprenylation synergizes with MAPK blockade to prevent growth in treatment-resistant melanoma, colorectal, and lung cancer. Pigment Cell Melanoma Res :
Senft, Daniela; Qi, Jianfei; Ronai, Ze'ev A (2018) Ubiquitin ligases in oncogenic transformation and cancer therapy. Nat Rev Cancer 18:69-88
Liu, Xiaoni; Zhang, Shang-Min; McGeary, Meaghan K et al. (2018) KDM5B Promotes Drug Resistance by Regulating Melanoma Propagating Cell Subpopulations. Mol Cancer Ther :
Pathria, Gaurav; Scott, David A; Feng, Yongmei et al. (2018) Targeting the Warburg effect via LDHA inhibition engages ATF4 signaling for cancer cell survival. EMBO J 37:
Wang, Jake; Perry, Curtis J; Meeth, Katrina et al. (2017) UV-induced somatic mutations elicit a functional T cell response in the YUMMER1.7 mouse melanoma model. Pigment Cell Melanoma Res 30:428-435
Falletta, Paola; Sanchez-Del-Campo, Luis; Chauhan, Jagat et al. (2017) Translation reprogramming is an evolutionarily conserved driver of phenotypic plasticity and therapeutic resistance in melanoma. Genes Dev 31:18-33
Theodosakis, Nicholas; Micevic, Goran; Langdon, Casey G et al. (2017) p90RSK Blockade Inhibits Dual BRAF and MEK Inhibitor-Resistant Melanoma by Targeting Protein Synthesis. J Invest Dermatol 137:2187-2196
Damsky, W E; Bosenberg, M (2017) Melanocytic nevi and melanoma: unraveling a complex relationship. Oncogene 36:5771-5792
Poothong, Juthakorn; Sopha, Pattarawut; Kaufman, Randal J et al. (2017) IRE1? nucleotide sequence cleavage specificity in the unfolded protein response. FEBS Lett 591:406-414

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