The DNA Synthesis Resource Core plays an essential role in the successful integration of this Program Project by providing high purity, site-specifically modified oligonucleotides to Projects 1, 2 and 3. The DNA Synthesis Resource Core works closely with Project 1, where novel reagents and synthetic strategies are developed for the site-specific synthesis of oligonucleotides containing the Fapy-dG lesions of interest to the Program ProjecL The technology is transferred to the Core, where the desired modified oligonucleotides are synthesized on the appropriate scale and purity for further chemical and replication studies by Project 1, cell-based studies by Project 2, and structural studies by Project 3.
The Specific Aims for the DNA Synthesis Resource Core are: 1) to synthesize the phosphoramidite reagents of the various Fapy-dG lesions and Incorporate them into oligonucleotides;2) develop protocols for the purification of the modified oligonucleotides;and 3) provide initial characterization of modified oligonucleotide.

National Institute of Health (NIH)
National Cancer Institute (NCI)
Research Program Projects (P01)
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Special Emphasis Panel (ZCA1-RPRB-O (M1))
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Vanderbilt University Medical Center
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Lin, Ying-Chih; Li, Liang; Makarova, Alena V et al. (2014) Molecular basis of aflatoxin-induced mutagenesis-role of the aflatoxin B1-formamidopyrimidine adduct. Carcinogenesis 35:1461-8
Lin, Ying-Chih; Li, Liang; Makarova, Alena V et al. (2014) Error-prone replication bypass of the primary aflatoxin B1 DNA adduct, AFB1-N7-Gua. J Biol Chem 289:18497-506
Minko, Irina G; Earley, Lauriel F; Larlee, Kimberly E et al. (2014) Pyrosequencing: applicability for studying DNA damage-induced mutagenesis. Environ Mol Mutagen 55:601-8
Christov, Plamen P; Son, Kyu-Jun; Rizzo, Carmelo J (2014) Synthesis and characterization of oligonucleotides containing a nitrogen mustard formamidopyrimidine monoadduct of deoxyguanosine. Chem Res Toxicol 27:1610-8
Singh, Vipender; Fedeles, Bogdan I; Li, Deyu et al. (2014) Mechanism of repair of acrolein- and malondialdehyde-derived exocyclic guanine adducts by the ?-ketoglutarate/Fe(II) dioxygenase AlkB. Chem Res Toxicol 27:1619-31
Gruppi, Francesca; Salyard, Tracy L Johnson; Rizzo, Carmelo J (2014) Synthesis of G-N (2)-(CH2)3-N (2)-G Trimethylene DNA interstrand cross-links. Curr Protoc Nucleic Acid Chem 5:5.14.1-5.14.15
Patra, Amritraj; Nagy, Leslie D; Zhang, Qianqian et al. (2014) Kinetics, structure, and mechanism of 8-Oxo-7,8-dihydro-2'-deoxyguanosine bypass by human DNA polymerase ?. J Biol Chem 289:16867-82
Balbo, Silvia; Turesky, Robert J; Villalta, Peter W (2014) DNA adductomics. Chem Res Toxicol 27:356-66
Petrova, Katya V; Millsap, Amy D; Stec, Donald F et al. (2014) Characterization of the deoxyguanosine-lysine cross-link of methylglyoxal. Chem Res Toxicol 27:1019-29
Gold, Barry; Stone, Michael P; Marky, Luis A (2014) Looking for Waldo: a potential thermodynamic signature to DNA damage. Acc Chem Res 47:1446-54

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