The DNA Synthesis Resource Core plays an essential role in the successful integration of this Program Project by providing high purity, site-specifically modified oligonucleotides to Projects 1, 2 and 3. The DNA Synthesis Resource Core works closely with Project 1, where novel reagents and synthetic strategies are developed for the site-specific synthesis of oligonucleotides containing the Fapy-dG lesions of interest to the Program ProjecL The technology is transferred to the Core, where the desired modified oligonucleotides are synthesized on the appropriate scale and purity for further chemical and replication studies by Project 1, cell-based studies by Project 2, and structural studies by Project 3.
The Specific Aims for the DNA Synthesis Resource Core are: 1) to synthesize the phosphoramidite reagents of the various Fapy-dG lesions and Incorporate them into oligonucleotides;2) develop protocols for the purification of the modified oligonucleotides;and 3) provide initial characterization of modified oligonucleotide.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Program Projects (P01)
Project #
2P01CA160032-21A1
Application #
8369644
Study Section
Special Emphasis Panel (ZCA1-RPRB-O (M1))
Project Start
1997-08-01
Project End
2017-07-31
Budget Start
2012-09-20
Budget End
2013-07-31
Support Year
21
Fiscal Year
2012
Total Cost
$85,349
Indirect Cost
$20,354
Name
Vanderbilt University Medical Center
Department
Type
DUNS #
004413456
City
Nashville
State
TN
Country
United States
Zip Code
37212
Patra, Amritraj; Politica, Dustin A; Chatterjee, Arindom et al. (2016) Mechanism of Error-Free Bypass of the Environmental Carcinogen N-(2'-Deoxyguanosin-8-yl)-3-aminobenzanthrone Adduct by Human DNA Polymerase η. Chembiochem 17:2033-2037
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Patra, Amitraj; Zhang, Qianqian; Guengerich, F Peter et al. (2016) Mechanisms of Insertion of dCTP and dTTP Opposite the DNA Lesion O6-Methyl-2'-deoxyguanosine by Human DNA Polymerase η. J Biol Chem 291:24304-24313
O'Flaherty, Derek K; Guengerich, F Peter; Egli, Martin et al. (2015) Backbone Flexibility Influences Nucleotide Incorporation by Human Translesion DNA Polymerase η opposite Intrastrand Cross-Linked DNA. Biochemistry 54:7449-56

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