The three research projects that comprise this Program Project are all focused on understanding molecular and cellular events and mechanisms leading to bladder carcinogenesis of both major pathways of low-grade, non-invasive and high-grade, invasive bladder cancer. In addition t o t h e intellectual and experimental synergies that link the individual projects into a cohesive program, each ofthe three proposed research plans all depend upon a common set of critical reagents and services made available on a continuous basis for their success. These include but are not limited to a set of cultured cell lines that represent different stages of bladder cancer, different unique mouse models of bladder cancer developed by Dr. Xue-Ru Wu, recombinant proteins, nucleic acid reagents, antibodies and cDNA constructs. Obviously, it would not make sense, nor would It be an efficient use of time, money, and resources for each laboratory to Independently generate, procure, characterize, and maintain all these reagents. Instead, we will establish a centralized Reagent and Service Core, one of whose purposes is to serve as a central repository for key reagents and provide a central supply for these important research tools that can be shared by all involved in this Program Project. In addition to the cost-effectiveness, this approach offers the advantage of ensuring the quality of these shared reagents across the individual projects. Providing supplies of cell lines, tissues and animal models from a centralized core eliminates variability in reagent handling, facilitating data comparison amongst the three individual projects. Furthermore, it promotes additional interactions among the Pis and guards against accidental loss of Invaluable reagents in emergency situations such as power outages (which we last experienced in 2003). Finally, the Core will also provide pathology and statistical support for the individual projects. In this section ofthe Program Project application, we describe these reagents, how the Core functions to control quality and maintain databases, the contribution ofthe core to the individual research projects, and a plan for prioritizing usage of core services.
The specific aims ofthe Core are: i) To maintain a centralized supply and distribute a common core set of reagents vital to support the research efforts of individual component projects;ii) To collect human and mouse specimens and provide the necessary pathology support service;and iii) To provide dedicated statistical support.

Public Health Relevance

;Bladder cancer (BC) remains a leading cause of death from cancer in both men and women. The three individual, yet inter-related research projects that make up this Program Project all seek to understand bladder cancer pathogenesis and the mechanisms involved as well. Besides being scientifically inter-related, the three projects depend upon a common set of reagents and services made available to them for their success. The Core bundles these critical reagents and services together and serves as a central clearinghouse, ensuring their timely, cost-effective distribution and direcfiy facilitating the Program's success.

National Institute of Health (NIH)
National Cancer Institute (NCI)
Research Program Projects (P01)
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New York University
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Zhang, Jingjie; Gao, Guangxun; Chen, Liang et al. (2014) Hydrogen peroxide/ATR-Chk2 activation mediates p53 protein stabilization and anti-cancer activity of cheliensisin A in human cancer cells. Oncotarget 5:841-52
Cao, Zipeng; Li, Xueyong; Li, Jingxia et al. (2014) X-linked inhibitor of apoptosis protein (XIAP) lacking RING domain localizes to the nuclear and promotes cancer cell anchorage-independent growth by targeting the E2F1/Cyclin E axis. Oncotarget 5:7126-37
Vieira, Neide; Deng, Fang-Ming; Liang, Feng-Xia et al. (2014) SNX31: a novel sorting nexin associated with the uroplakin-degrading multivesicular bodies in terminally differentiated urothelial cells. PLoS One 9:e99644
Zhang, Ruowen; Wang, Yulei; Li, Jingxia et al. (2014) The Chinese herb isolate yuanhuacine (YHL-14) induces G2/M arrest in human cancer cells by up-regulating p21 protein expression through an p53 protein-independent cascade. J Biol Chem 289:6394-403
Fang, Yong; Wang, Yihong; Wang, Yulei et al. (2014) A new tumour suppression mechanism by p27Kip1: EGFR down-regulation mediated by JNK/c-Jun pathway inhibition. Biochem J 463:383-92
Zhu, Junlan; Zhang, Jingjie; Huang, Haishan et al. (2014) Crucial role of c-Jun phosphorylation at Ser63/73 mediated by PHLPP protein degradation in the cheliensisin a inhibition of cell transformation. Cancer Prev Res (Phila) 7:1270-81
Madka, Venkateshwar; Mohammed, Altaf; Li, Qian et al. (2014) Chemoprevention of urothelial cell carcinoma growth and invasion by the dual COX-LOX inhibitor licofelone in UPII-SV40T transgenic mice. Cancer Prev Res (Phila) 7:708-16
Cao, Zipeng; Li, Xueyong; Li, Jingxia et al. (2014) SUMOylation of RhoGDI* is required for its repression of cyclin D1 expression and anchorage-independent growth of cancer cells. Mol Oncol 8:285-96
Huang, Haishan; Ma, Li; Li, Jingxia et al. (2014) NF-?B1 inhibits c-Myc protein degradation through suppression of FBW7 expression. Oncotarget 5:493-505
Gao, Guangxun; Chen, Liang; Li, Jingxia et al. (2014) Isorhapontigenin (ISO) inhibited cell transformation by inducing G0/G1 phase arrest via increasing MKP-1 mRNA Stability. Oncotarget 5:2664-77

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