The GhlP-Seq Production core will be responsible for 1) performing ChlP-assays;2) generating library for lllumina next-generation sequencing, 3) mapping sequence data to KSHV and human genomes, and 4) providing follow-up bioinformatics analyses. These goals will be. accomplished in conjunction with the Wistar Institutes Genomics and Bioinformatics Core, which provides lllumina Next Generation Sequencing as a core service. Final data management and analyses will be made accessible to each project investigator via a shared web-based browser for KSHV. Expertise in all aspects of ChIP, Next-Generation Sequencing, and Bioinformatic support will be provided to the projects for the lifetime of grant support. The GhlP-Seq core will be lead by Dr. Paul Lieberman, who is familler with ChIP Seq methods and bioinformatics analyses as applied to KSHV and EBV genomes. The ChlP-Seq core will facilitate the ability of all members of the Program project to standardize, methods for sample preparation and for post-sequence analysis. The work will be performed at the Wistar Institute in space within the Lieberman lab;and in conjunction with the Wistar Institute Genomics Core and Sequencing facility.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Program Projects (P01)
Project #
1P01CA174439-01A1
Application #
8541143
Study Section
Special Emphasis Panel (ZCA1-RPRB-2 (J1))
Project Start
2013-05-08
Project End
2018-04-30
Budget Start
2013-05-08
Budget End
2014-04-30
Support Year
1
Fiscal Year
2013
Total Cost
$324,873
Indirect Cost
$81,427
Name
University of Pennsylvania
Department
Type
DUNS #
042250712
City
Philadelphia
State
PA
Country
United States
Zip Code
19104
Shukla, Sanket Kumar; Jha, Hem Chandra; El-Naccache, Darine W et al. (2016) An EBV recombinant deleted for residues 130-159 in EBNA3C can deregulate p53/Mdm2 and Cyclin D1/CDK6 which results in apoptosis and reduced cell proliferation. Oncotarget 7:18116-34
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Sun, Zhiguo; Jha, Hem Chandra; Pei, Yong-Gang et al. (2016) Major Histocompatibility Complex Class II HLA-DRα Is Downregulated by Kaposi's Sarcoma-Associated Herpesvirus-Encoded Lytic Transactivator RTA and MARCH8. J Virol 90:8047-58
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Uppal, Timsy; Jha, Hem C; Verma, Subhash C et al. (2015) Chromatinization of the KSHV Genome During the KSHV Life Cycle. Cancers (Basel) 7:112-42
Jha, Hem C; Yang, Karren; El-Naccache, Darine W et al. (2015) EBNA3C regulates p53 through induction of Aurora kinase B. Oncotarget 6:5788-803
Tsai, Kevin; Messick, Troy E; Lieberman, Paul M (2015) Disruption of host antiviral resistances by gammaherpesvirus tegument proteins with homology to the FGARAT purine biosynthesis enzyme. Curr Opin Virol 14:30-40

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