The overall goal of this Program Project Group is to understand the mechanisms underlying renal fluid, electrolyte and macromolecule transport. Kinase and phosphatases are essential regulators of this process and thus, by extension, their target phosphorylation sites are important for mechanistic insight. This core will add value to the project group by bringing new capabilities and expertise in the identification and characterization of phosphorylation sites in proteins and proteomes. Dr. Jesse Rinehart will assume the role as the Phosphoproteomics Core Director and will bring expertise in phosphorylation mapping, quantitative proteomics, and phosphoprotein synthesis to the Program Project Group. Dr. Rinehart recently developed a new technology which enables site-specific incorporation of phosphoserine into proteins. This technology enables the synthesis of physiologically relevant phosphoproteins in an E. coli strain with an expanded genetic code. This unique technology and a recently published next- generation phosphoserine technology will be uniquely available to this Program Project Group. The combinations of established phosphoproteomics technologies and novel phosphoprotein synthesis technologies will enable a unique approach to validation and further exploration of the mechanisms of protein phosphorylation. The core will be an excellent learning environment and bring trainees from the Program Project Groups in close contact with proteomics experts in the Rinehart lab. This training environment will provide access to state-of-the-art technology to the individual members of the Project Group. Dr. Rinehart's research interests are closely aligned with the aims of this project group and, in addition to the technology, the unique lab environment is value added to the Program Project Group.
The purpose of this Phosphoproteomics Core is to provide a fundamental connection to the understanding of protein phosphorylation and the mechanisms that coordinate electrolyte homeostasis. This knowledge is essential and will benefit human health.
|Kim, Jun-Mo; Xu, Shuhua; Guo, Xiaoyun et al. (2018) Urinary bladder hypertrophy characteristic of male ROMK Bartter's mice does not occur in female mice. Am J Physiol Regul Integr Comp Physiol 314:R334-R341|
|Gassaway, Brandon M; Petersen, Max C; Surovtseva, Yulia V et al. (2018) PKC? contributes to lipid-induced insulin resistance through cross talk with p70S6K and through previously unknown regulators of insulin signaling. Proc Natl Acad Sci U S A 115:E8996-E9005|
|Gilder, Allison L; Chapin, Hannah C; Padovano, Valeria et al. (2018) Newly synthesized polycystin-1 takes different trafficking pathways to the apical and ciliary membranes. Traffic 19:933-945|
|Barber, Karl W; Muir, Paul; Szeligowski, Richard V et al. (2018) Encoding human serine phosphopeptides in bacteria for proteome-wide identification of phosphorylation-dependent interactions. Nat Biotechnol 36:638-644|
|Scholl, Ute I; Stölting, Gabriel; Schewe, Julia et al. (2018) CLCN2 chloride channel mutations in familial hyperaldosteronism type II. Nat Genet 50:349-354|
|Barber, Karl W; Rinehart, Jesse (2018) The ABCs of PTMs. Nat Chem Biol 14:188-192|
|Barber, Karl W; Miller, Chad J; Jun, Jay W et al. (2018) Kinase Substrate Profiling Using a Proteome-wide Serine-Oriented Human Peptide Library. Biochemistry 57:4717-4725|
|Castañeda-Bueno, Maria; Arroyo, Juan Pablo; Zhang, Junhui et al. (2017) Phosphorylation by PKC and PKA regulate the kinase activity and downstream signaling of WNK4. Proc Natl Acad Sci U S A 114:E879-E886|
|Mohler, Kyle; Aerni, Hans-Rudolf; Gassaway, Brandon et al. (2017) MS-READ: Quantitative measurement of amino acid incorporation. Biochim Biophys Acta Gen Subj 1861:3081-3088|
|D'Lima, Nadia G; Khitun, Alexandra; Rosenbloom, Aaron D et al. (2017) Comparative Proteomics Enables Identification of Nonannotated Cold Shock Proteins in E. coli. J Proteome Res 16:3722-3731|
Showing the most recent 10 out of 303 publications