Imaging techniques constitute critical tools that are brought to bear with creativity and power in many aspects of modern renal research. The renal researchers who participate in this Program Project Grant require ready access to high quality imaging equipment and services. The existing Microscopy and Imaging Center core facility has been designed to meet this need. The core has been structured in a manner that unites resources and expertise from several different locations within the University into a single unified entity that can serve as an integral partner in the design and execution of imaging studies. The facility includes a full range of high quality imaging equipment, including: fluorescence photomicroscopes, fluorescence imaging systems, a new laser scanning confocal microscope, a spinning disk confocal microscope, an electron microscope and numerous devices for tissue sectioning and sample preparation. The technical staff of the core manifests decades of combined experience in imaging methodologies and technique development. The supervisory staff includes two senior scientists, both of whom have extensive expertise in the application of the various modalities of imaging to renal and physiological research. This team will work together to meet the following objectives: 1) to provide PPG group researchers with ready access to high quality imaging modalities, including fluorescence microscopy, laser scanning confocal microscopy, real time imagining of physiological parameters in cells and tubules and electron microscopy;2) to provide PPG group researchers with expert sample preparation and processing for light and electron microscopy;3) to provide PPG group researchers with expert assistance in the generation, interpretation and quantitation of static images, including those documenting organ structure, renal histology, and the subcellular localizations of renal proteins;and 4) to provide PPG group researchers with access to the equipment and expertise required to produce dynamic images of living cells and tissues, including in vivo assessment of organ structure, measurements of intracellular ion concentrations, and real time observations of protein trafficking.

Public Health Relevance

Imaging techniques play a critical role in modern renal research. The Microscopy and Imaging Center core facility will provide participants in the Program Project with access to all of the equipment required to perform sophisticated imaging analysis of renal cells and tissues. Most importantly, it will also provide researchers with access to expert guidance and technical assistance for sample preparation and image analysis.

National Institute of Health (NIH)
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Research Program Projects (P01)
Project #
Application #
Study Section
Special Emphasis Panel (ZDK1-GRB-9 (M6))
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Yale University
New Haven
United States
Zip Code
Mohler, Kyle; Aerni, Hans-Rudolf; Gassaway, Brandon et al. (2017) MS-READ: Quantitative measurement of amino acid incorporation. Biochim Biophys Acta 1861:3081-3088
Castañeda-Bueno, Maria; Arroyo, Juan Pablo; Zhang, Junhui et al. (2017) Phosphorylation by PKC and PKA regulate the kinase activity and downstream signaling of WNK4. Proc Natl Acad Sci U S A 114:E879-E886
Han, Jaeyong; Lee, Seung Hun; Giebisch, Gerhard et al. (2016) Potassium Channelopathies and Gastrointestinal Ulceration. Gut Liver 10:881-889
Stoops, Emily H; Hull, Michael; Caplan, Michael J (2016) Newly synthesized and recycling pools of the apical protein gp135 do not occupy the same compartments. Traffic 17:1272-1285
Ferdaus, Mohammed Z; Barber, Karl W; López-Cayuqueo, Karen I et al. (2016) SPAK and OSR1 play essential roles in potassium homeostasis through actions on the distal convoluted tubule. J Physiol 594:4945-66
Ishizawa, Kenichi; Xu, Ning; Loffing, Johannes et al. (2016) Potassium depletion stimulates Na-Cl cotransporter via phosphorylation and inactivation of the ubiquitin ligase Kelch-like 3. Biochem Biophys Res Commun 480:745-751
Lin, Dao-Hong; Yue, Peng; Yarborough 3rd, Orlando et al. (2015) Src-family protein tyrosine kinase phosphorylates WNK4 and modulates its inhibitory effect on KCNJ1 (ROMK). Proc Natl Acad Sci U S A 112:4495-500
Chen, Tiane; Kocinsky, Hetal S; Cha, Boyoung et al. (2015) Cyclic GMP kinase II (cGKII) inhibits NHE3 by altering its trafficking and phosphorylating NHE3 at three required sites: identification of a multifunctional phosphorylation site. J Biol Chem 290:1952-65
Pirman, Natasha L; Barber, Karl W; Aerni, Hans R et al. (2015) A flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation. Nat Commun 6:8130
Farr, Glen A; Hull, Michael; Stoops, Emily H et al. (2015) Dual pulse-chase microscopy reveals early divergence in the biosynthetic trafficking of the Na,K-ATPase and E-cadherin. Mol Biol Cell 26:4401-11

Showing the most recent 10 out of 290 publications