Imaging techniques constitute critical tools that are brought to bear with creativity and power in many aspects of modern renal research. The renal researchers who participate in this Program Project Grant require ready access to high quality imaging equipment and services. The existing Microscopy and Imaging Center core facility has been designed to meet this need. The core has been structured in a manner that unites resources and expertise from several different locations within the University into a single unified entity that can serve as an integral partner in the design and execution of imaging studies. The facility includes a full range of high quality imaging equipment, including: fluorescence photomicroscopes, fluorescence imaging systems, a new laser scanning confocal microscope, a spinning disk confocal microscope, an electron microscope and numerous devices for tissue sectioning and sample preparation. The technical staff of the core manifests decades of combined experience in imaging methodologies and technique development. The supervisory staff includes two senior scientists, both of whom have extensive expertise in the application of the various modalities of imaging to renal and physiological research. This team will work together to meet the following objectives: 1) to provide PPG group researchers with ready access to high quality imaging modalities, including fluorescence microscopy, laser scanning confocal microscopy, real time imagining of physiological parameters in cells and tubules and electron microscopy;2) to provide PPG group researchers with expert sample preparation and processing for light and electron microscopy;3) to provide PPG group researchers with expert assistance in the generation, interpretation and quantitation of static images, including those documenting organ structure, renal histology, and the subcellular localizations of renal proteins;and 4) to provide PPG group researchers with access to the equipment and expertise required to produce dynamic images of living cells and tissues, including in vivo assessment of organ structure, measurements of intracellular ion concentrations, and real time observations of protein trafficking.

Public Health Relevance

Imaging techniques play a critical role in modern renal research. The Microscopy and Imaging Center core facility will provide participants in the Program Project with access to all of the equipment required to perform sophisticated imaging analysis of renal cells and tissues. Most importantly, it will also provide researchers with access to expert guidance and technical assistance for sample preparation and image analysis.

National Institute of Health (NIH)
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Research Program Projects (P01)
Project #
Application #
Study Section
Special Emphasis Panel (ZDK1-GRB-9 (M6))
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Yale University
New Haven
United States
Zip Code
Monette, Michelle Y; Somasekharan, Suma; Forbush, Biff (2014) Molecular motions involved in Na-K-Cl cotransporter-mediated ion transport and transporter activation revealed by internal cross-linking between transmembrane domains 10 and 11/12. J Biol Chem 289:7569-79
Merrick, David; Bertuccio, Claudia A; Chapin, Hannah C et al. (2014) Polycystin-1 cleavage and the regulation of transcriptional pathways. Pediatr Nephrol 29:505-11
Stoops, Emily H; Caplan, Michael J (2014) Trafficking to the apical and basolateral membranes in polarized epithelial cells. J Am Soc Nephrol 25:1375-86
Stoops, Emily H; Farr, Glen A; Hull, Michael et al. (2014) SNAP-tag to monitor trafficking of membrane proteins in polarized epithelial cells. Methods Mol Biol 1174:171-82
Shibata, Shigeru; Arroyo, Juan Pablo; Castañeda-Bueno, María et al. (2014) Angiotensin II signaling via protein kinase C phosphorylates Kelch-like 3, preventing WNK4 degradation. Proc Natl Acad Sci U S A 111:15556-61
Hayashi, Hisayoshi; Tamura, Atsushi; Krishnan, Devishree et al. (2013) Ezrin is required for the functional regulation of the epithelial sodium proton exchanger, NHE3. PLoS One 8:e55623
Shibata, Shigeru; Rinehart, Jesse; Zhang, Junhui et al. (2013) Mineralocorticoid receptor phosphorylation regulates ligand binding and renal response to volume depletion and hyperkalemia. Cell Metab 18:660-71
Shibata, Shigeru; Zhang, Junhui; Puthumana, Jeremy et al. (2013) Kelch-like 3 and Cullin 3 regulate electrolyte homeostasis via ubiquitination and degradation of WNK4. Proc Natl Acad Sci U S A 110:7838-43
Jouret, François; Wu, Jingshing; Hull, Michael et al. (2013) Activation of the Ca²+-sensing receptor induces deposition of tight junction components to the epithelial cell plasma membrane. J Cell Sci 126:5132-42
Wells, Erika K; Yarborough 3rd, OrLando; Lifton, Richard P et al. (2013) Epithelial morphogenesis of MDCK cells in three-dimensional collagen culture is modulated by interleukin-8. Am J Physiol Cell Physiol 304:C966-75

Showing the most recent 10 out of 271 publications