Core B has continuously served all projects of the PPG for the past 18 years of funding. The functions of this core in this renewal application are divided into three divisions. Division I is responsible for the acquisition, breeding and maintenance of mouse colonies, the predominant species used for this project. This division also provides fresh cells and tissues to the various projects as well as culturing both cells and tissues. In addition to mice, Division I is also involved in the acquisition of gastrointestinal muscle samples from the Cyonomolgus monkey and from patients undergoing either gastric bypass surgery or surgery for colon cancer. In the last funding cycle a second division was added to the core, namely flow cytometry. The flow cytometry division (Division II) has been extremely successful in sorting and analyzing various populations of cells. Our recent acquisition of transgenic mice expressing either eGFP in smooth muscle cells (smMHC-cre-eGFP) or copGFP in ICC (Kit+/copGFP) has further advanced our ability to sort and analyze highly pure populations of these two cells types. These models have also been instrumental in improving our ability to reliably isolate and study either smooth muscle or ICC specific proteins. In this current funding cycle we have added a third subdivision to core B which is focused upon the generation of new transgenic mouse models (Division III). This division has already successfully generated the copGFP ICC mouse model described above and will provide the various projects with additional ICC- or smooth muscle-specific knockout or knock-in mouse models to address the specific aims of their projects. In summary, Core B is an essential and highly successful component of the PPG which continues to grow and diversify as new methodologies and approaches become available.

Public Health Relevance

(Seeinstructions): Core B supplies animals, cells and tissues to the various projects.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Program Projects (P01)
Project #
5P01DK041315-25
Application #
8469495
Study Section
Special Emphasis Panel (ZDK1-GRB-9)
Project Start
Project End
Budget Start
2013-05-01
Budget End
2014-04-30
Support Year
25
Fiscal Year
2013
Total Cost
$282,614
Indirect Cost
$81,465
Name
University of Nevada Reno
Department
Type
DUNS #
146515460
City
Reno
State
NV
Country
United States
Zip Code
89557
Baker, Salah A; Drumm, Bernard T; Saur, Dieter et al. (2016) Spontaneous Ca(2+) transients in interstitial cells of Cajal located within the deep muscular plexus of the murine small intestine. J Physiol 594:3317-38
Durnin, Leonie; Hayoz, Sebastien; Corrigan, Robert D et al. (2016) Urothelial purine release during filling of murine and primate bladders. Am J Physiol Renal Physiol 311:F708-F716
Sanders, Kenton M; Ward, Sean M; Friebe, Andreas (2016) Rebuttal from Kenton M. Sanders, Sean M. Ward and Andreas Friebe. J Physiol 594:1515
Hwang, Sung Jin; Basma, Naseer; Sanders, Kenton M et al. (2016) Effects of new-generation inhibitors of the calcium-activated chloride channel anoctamin 1 on slow waves in the gastrointestinal tract. Br J Pharmacol 173:1339-49
Durnin, L; Moreland, N; Lees, A et al. (2016) A commonly used ecto-ATPase inhibitor, ARL-67156, blocks degradation of ADP more than the degradation of ATP in murine colon. Neurogastroenterol Motil 28:1370-81
Sanders, Kenton M; Ward, Sean M; Friebe, Andreas (2016) CrossTalk proposal: Interstitial cells are involved and physiologically important in neuromuscular transmission in the gut. J Physiol 594:1507-9
Sanders, Kenton M (2015) New Molecular Tools to Investigate the Development and Functions of Interstitial Cells of Cajal in the GI Tract. Gastroenterology 149:283-6
Peri, Lauren E; Koh, Byoung H; Ward, Grace K et al. (2015) A novel class of interstitial cells in the mouse and monkey female reproductive tracts. Biol Reprod 92:102
Baker, Salah A; Hennig, Grant W; Ward, Sean M et al. (2015) Temporal sequence of activation of cells involved in purinergic neurotransmission in the colon. J Physiol 593:1945-63
Zhu, Mei Hong; Sung, Tae Sik; O'Driscoll, Kate et al. (2015) Intracellular Ca(2+) release from endoplasmic reticulum regulates slow wave currents and pacemaker activity of interstitial cells of Cajal. Am J Physiol Cell Physiol 308:C608-20

Showing the most recent 10 out of 349 publications