Animal Model Core B will provide all mice used in Projects 1, 2 and 3, i.e., it will be responsible for ordering mice, performing the various manipulations required to induce experimental colitis, maintaining a barrier facility, performing routine surveys to ensure absence of pathogens in the colony. And assist in harvesting tissues from such mice. Centralization of these functions in an Animal Model Core provides uniformity and quality control of mice being studied in the various projects, reduces cost, and should help conserve numbers of mice required by ensuring their optimal use in the projects.
A second aim of the Animal Model Core is that of a centralized pathologic analyses which will be performed by Dr. Audrey Lazenby, an expert GI pathologist. Tissue sections from the various projects are coded prior to interpretation and quantitative scoring. This facilitates interactions among the projects and also the comparison of results between them. A third purpose of the Animal Model Core, which is new since the last submission, is to provide genetically modified transgenic and knockout mouse strains for use in experiments in the various projects. Such genetically altered strains are powerful analytic tools for defining the role of specific gene products in complex biologic systems that are becoming the """"""""reagents"""""""" that allow fundamental questions to be experimentally addressed that had been previously unapproachable. Mice with induced mutations frequently have to be bred onto other background strains for optimal study, which requires screening of individual animals in each generation of identification of one or more target genetic elements. Approximately 30 mutant or genetically engineered strains are currently maintained in the Core. Dr. Charles Elson will serve as Director and Dr. Casey Weaver will serve as Co-Director of this Core. Mice are housed under barrier conditions in two locations, the 8th floor of the Lyons Harrison Research Building and the Animal Facility in the Wallace Tumor Institute. Dr. J. Russell Lindsay and Dr. Carl Pinkert from the Department of Comparative Medicine will serve as Consultants. An Oversight Committee consisting of the PI of each project with Dr. Lindsay and Dr. Pinkert will oversee the usage and function of the Core.

Project Start
2000-09-01
Project End
2001-08-31
Budget Start
Budget End
Support Year
10
Fiscal Year
2000
Total Cost
$154,165
Indirect Cost
Name
University of Alabama Birmingham
Department
Type
DUNS #
004514360
City
Birmingham
State
AL
Country
United States
Zip Code
35294
Jurisic, Giorgia; Sundberg, John P; Detmar, Michael (2013) Blockade of VEGF receptor-3 aggravates inflammatory bowel disease and lymphatic vessel enlargement. Inflamm Bowel Dis 19:1983-9
Luckey, C John; Weaver, Casey T (2012) Stem-cell-like qualities of immune memory; CD4+ T cells join the party. Cell Stem Cell 10:107-8
Jurisic, G; Sundberg, J P; Bleich, A et al. (2010) Quantitative lymphatic vessel trait analysis suggests Vcam1 as candidate modifier gene of inflammatory bowel disease. Genes Immun 11:219-31
Bleich, Andre; Büchler, Gwen; Beckwith, Jason et al. (2010) Cdcs1 a major colitis susceptibility locus in mice; subcongenic analysis reveals genetic complexity. Inflamm Bowel Dis 16:765-75
Maynard, Craig L; Weaver, Casey T (2008) Diversity in the contribution of interleukin-10 to T-cell-mediated immune regulation. Immunol Rev 226:219-33
Elson, Charles O; Cong, Yingzi; Weaver, Casey T et al. (2007) Monoclonal anti-interleukin 23 reverses active colitis in a T cell-mediated model in mice. Gastroenterology 132:2359-70
Duverger, Alexandra; Jackson, Raymond J; van Ginkel, Frederick W et al. (2006) Bacillus anthracis edema toxin acts as an adjuvant for mucosal immune responses to nasally administered vaccine antigens. J Immunol 176:1776-83
Elson, Charles O; Cong, Yingzi; Qi, Fengxia et al. (2006) Molecular approaches to the role of the microbiota in inflammatory bowel disease. Ann N Y Acad Sci 1072:39-51
Konrad, Astrid; Cong, Yingzi; Duck, Wayne et al. (2006) Tight mucosal compartmentation of the murine immune response to antigens of the enteric microbiota. Gastroenterology 130:2050-9
van Ginkel, Frederik W; Jackson, Raymond J; Yoshino, Naoto et al. (2005) Enterotoxin-based mucosal adjuvants alter antigen trafficking and induce inflammatory responses in the nasal tract. Infect Immun 73:6892-902

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