The incidence of diabetes in the United States is increasing rapidly as a result of the obesity epidemic. Mouse models have increased our understanding of the pathogenesis of diabetes and other metabolic diseases. The projects described in this grant ufilize various genefic models to study the molecular mechanisms underiying energy homeostasis, and glucose and lipid metabolism. Core D provides accurate, timely and cost-effective phenotyping of mouse models generated by the Pis. Core D is directed by the PI of Project 3, Rex Ahima, who is also the director of the Penn Diabetes and Endocrinology Research Center (DERC) Mouse Phenotyping, Physiology and Metabolism Core. Core D supports the salary of a research specialist, supervised by the director Rex Ahima, to perform in vivo metabolic studies in mice. Core D uses state-of-the-art equipment and in vivo techniques. A Comprehensive Laboratory Animal Monitoring System (CLAMS) is used for assessment of food intake, drinking, energy expenditure, locomotor activity and sleep epochs. Nuclear Magnefic Resonance (NMR) and Dual Emission Xray Absorpfiometry (DEXA) are used for assessment of body composifion. Glucose homeostasis is evaluated with glucose and insulin tolerance tests, and insulin clamp and radioisotopic tracer kinetics. Core D also performs treadmill exercise, infrared thermography, blood pressure and heart rate monitoring, analysis of tissue chemistry, and tracer studies in isolated organs.
Core D performs critical metabolic studies in mice generated by the Pis of this program, who lack the expertise or facilities to conduct these studies. Core D is directed by the PI of Project 3, Rex Ahima, and supporits the salary of a research specialist. The goal of Core D is to expedite the perfonnance of in vivo metabolic assays, ensure accuracy, and facilitate interactions among the Pis of the program project.
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|Soleimanpour, Scott A; Gupta, Aditi; Bakay, Marina et al. (2014) The diabetes susceptibility gene Clec16a regulates mitophagy. Cell 157:1577-90|
|Jiang, Shaoning; Park, Dae Won; Tadie, Jean-Marc et al. (2014) Human resistin promotes neutrophil proinflammatory activation and neutrophil extracellular trap formation and increases severity of acute lung injury. J Immunol 192:4795-803|
|Lee, Dolim; Le Lay, John; Kaestner, Klaus H (2014) The transcription factor CREB has no non-redundant functions in hepatic glucose metabolism in mice. Diabetologia 57:1242-8|
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|Baur, Joseph A; Birnbaum, Morris J (2014) Control of gluconeogenesis by metformin: does redox trump energy charge? Cell Metab 20:197-9|
|Shin, Soona; Kaestner, Klaus H (2014) The origin, biology, and therapeutic potential of facultative adult hepatic progenitor cells. Curr Top Dev Biol 107:269-92|
|Roat, Regan; Rao, Vandana; Doliba, Nicolai M et al. (2014) Alterations of pancreatic islet structure, metabolism and gene expression in diet-induced obese C57BL/6J mice. PLoS One 9:e86815|
|Liew, Chong Wee; Assmann, Anke; Templin, Andrew T et al. (2014) Insulin regulates carboxypeptidase E by modulating translation initiation scaffolding protein eIF4G1 in pancreatic ? cells. Proc Natl Acad Sci U S A 111:E2319-28|
|Carr, Rotonya M; Peralta, Giselle; Yin, Xiaoyan et al. (2014) Absence of perilipin 2 prevents hepatic steatosis, glucose intolerance and ceramide accumulation in alcohol-fed mice. PLoS One 9:e97118|
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