The Analytical Core facility will provide an essential I ink between the structural and biochemical studies proposed and application to diabetes. Additionally, this facility will provide a centralized resource. that will exploit existing technologies, previously established in the Taylor, Scott, Newton, and Olefsky laboratories and the Multi-Scale Imaging Core to expand the number and types of assays available for each laboratory to study signaling in diabetes. Therefore, the core will provide an essential point of synergy where al 1 laboratories will work together and ask critical questions about the molecular mechanisms underlying the diabetic state. This core, overseen by Drs. Taylor and King, will consist of four functional sub-units;protein expression, crystallography, proteomics/phosphoproteomics, and peptide arrays. The Analytical Core will provide the researchers in this Program Project Grant with 1)so luble, purified, recombinant proteins for structure-function studies;2) a centralized resource for protein crystallization, data colle cation, phase determination, model building, and structure refinement;3) ma ss spectrometry services . to map phosphorylation sites on proteins, identify proteins from crude and purified cellular fractionation experiments, define temporal protein -protein interactions, and quantitate protein expression;and 4) p eptide array technology to probe proteins of interest in molecular detail t o define interaction sites between proteins and generate 'super-binder'peptides? that can be used to manipulate signaling.

Public Health Relevance

The Analytical Core has traditionally provided a centralized point of synergy to the individual laboratories of this Program Project Grant and provided researchers access to specialized techniques that have accelerated the research and been inst rumental in the generation of no vel tools fo r the greater biological community. In the coming funding period, we have streamlined our services to provide better access of the laboratories to purified proteins, protein crystallization resources, a variety of mass spectrometry techniques, and peptide arrays.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Program Projects (P01)
Project #
2P01DK054441-14A1
Application #
8573099
Study Section
Special Emphasis Panel (ZDK1-GRB-6 (J1))
Project Start
Project End
Budget Start
2013-04-01
Budget End
2014-03-31
Support Year
14
Fiscal Year
2013
Total Cost
$160,427
Indirect Cost
$56,926
Name
University of California San Diego
Department
Type
DUNS #
804355790
City
La Jolla
State
CA
Country
United States
Zip Code
92093
Baeza-Raja, Bernat; Sachs, Benjamin D; Li, Pingping et al. (2016) p75 Neurotrophin Receptor Regulates Energy Balance in Obesity. Cell Rep 14:255-68
Cherry, Allison E; Haas, Brian R; Naydenov, Alipi V et al. (2016) ST-11: A New Brain-Penetrant Microtubule-Destabilizing Agent with Therapeutic Potential for Glioblastoma Multiforme. Mol Cancer Ther 15:2018-29
Turnham, Rigney E; Scott, John D (2016) Protein kinase A catalytic subunit isoform PRKACA; History, function and physiology. Gene 577:101-8
Nygren, Patrick J; Scott, John D (2016) Regulation of the phosphatase PP2B by protein-protein interactions. Biochem Soc Trans 44:1313-1319
Bjerregaard-Andersen, Kaare; Østensen, Ellen; Scott, John D et al. (2016) Malonate in the nucleotide-binding site traps human AKAP18γ/δ in a novel conformational state. Acta Crystallogr F Struct Biol Commun 72:591-7
Whiting, Jennifer L; Ogier, Leah; Forbush, Katherine A et al. (2016) AKAP220 manages apical actin networks that coordinate aquaporin-2 location and renal water reabsorption. Proc Natl Acad Sci U S A 113:E4328-37
Nieves-Cintrón, Madeline; Hirenallur-Shanthappa, Dinesh; Nygren, Patrick J et al. (2016) AKAP150 participates in calcineurin/NFAT activation during the down-regulation of voltage-gated K(+) currents in ventricular myocytes following myocardial infarction. Cell Signal 28:733-40
Kountz, Timothy S; Lee, Kyung-Soon; Aggarwal-Howarth, Stacey et al. (2016) Endogenous N-terminal Domain Cleavage Modulates α1D-Adrenergic Receptor Pharmacodynamics. J Biol Chem 291:18210-21
Cordes, Thekla; Wallace, Martina; Michelucci, Alessandro et al. (2016) Immunoresponsive Gene 1 and Itaconate Inhibit Succinate Dehydrogenase to Modulate Intracellular Succinate Levels. J Biol Chem 291:14274-84
Riggle, Kevin M; Riehle, Kimberly J; Kenerson, Heidi L et al. (2016) Enhanced cAMP-stimulated protein kinase A activity in human fibrolamellar hepatocellular carcinoma. Pediatr Res 80:110-8

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