Core A measures serum, serum ultrafiltrate (UF), plasma and urine stone risk chemistries and calculates urine supersaturations (SS) for patients studied in Projects 1 and 2. These measurements are used for fulfillment of aims within all three projects and six program aims carried out within the overall program project. Plasma GLP-1, renin, and plasma and urine cAMP will also be measured by Core A for Project 1. Blood and urine samples are processed and shipped for assay of lithium done in Core B and for measurement of plasma glucagon, parathyroid hormone (PTH) and 1,25 vitamin D (performed by LabCorp). Sample aliquots are stored at -80?C for future use. All laboratory data from Core A for all projects are consolidated into a single database with daily backup for security and ease of analysis. Ultimately, data from the other projects and cores (e.g. histology, biopsy, stone analysis etc.) will be consolidated into our database so that it can be analyzed along with serum and urine data.

Public Health Relevance

Core A performs blood and urine assays to characterize the metabolic abnormalities in all patients biopsied in Project 2. Core A also performs assays for studies of renal handling of minerals in stone formers and controls in Project 1, especially changes associated with eating, in order to uncover the abnormalities of renal calcium reabsorption that promote both calcium stone formation and renal papillary calcifications.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Program Projects (P01)
Project #
5P01DK056788-14
Application #
8730116
Study Section
Special Emphasis Panel (ZDK1-GRB-R)
Project Start
Project End
Budget Start
2014-08-01
Budget End
2015-07-31
Support Year
14
Fiscal Year
2014
Total Cost
$229,079
Indirect Cost
$82,151
Name
University of Chicago
Department
Type
DUNS #
005421136
City
Chicago
State
IL
Country
United States
Zip Code
60637
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Worcester, Elaine M; Bergsland, Kristin J; Gillen, Daniel L et al. (2013) Evidence for increased renal tubule and parathyroid gland sensitivity to serum calcium in human idiopathic hypercalciuria. Am J Physiol Renal Physiol 305:F853-60
Lingeman, James E (2013) Pathogenesis of nephrolithiasis. J Urol 189:417-8

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