The Cell and Molecular Biology Core (Core B) will provide reagents to assist in the study of nuclear calcium signaling and its effects in liver. The core will generate, purify and maintain adenoviruses and genetically engineered cell lines in which nuclear or cytosolic calcium signaling is suppressed. The core also will assist in the development and characterization of siRNA's requested for use in the individual projects. The core will provide hepatocytes and other cells from MKP-1 knock-out mice and transgenic mice expressing liverspecific expression of nuclear and cytosolic calcium buffering proteins for the analysis of calcium signaling and related studies on growth and metabolism. The core will include dedicated personnel and will be located with the Director's laboratory.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Program Projects (P01)
Project #
2P01DK057751-11
Application #
8146712
Study Section
Special Emphasis Panel (ZDK1-GRB-8 (J1))
Project Start
Project End
Budget Start
2011-08-02
Budget End
2012-04-30
Support Year
11
Fiscal Year
2011
Total Cost
$233,304
Indirect Cost
Name
Yale University
Department
Type
DUNS #
043207562
City
New Haven
State
CT
Country
United States
Zip Code
06520
Yang, Xiaoyong; Qian, Kevin (2017) Protein O-GlcNAcylation: emerging mechanisms and functions. Nat Rev Mol Cell Biol 18:452-465
Feriod, Colleen N; Oliveira, Andre Gustavo; Guerra, Mateus T et al. (2017) Hepatic Inositol 1,4,5 Trisphosphate Receptor Type 1 Mediates Fatty Liver. Hepatol Commun 1:23-35
Kruglov, Emma; Ananthanarayanan, Meenakshisundaram; Sousa, Pedro et al. (2017) Type 2 inositol trisphosphate receptor gene expression in hepatocytes is regulated by cyclic AMP. Biochem Biophys Res Commun 486:659-664
Wang, Simeng; Yang, Xiaoyong (2017) Inter-organ regulation of adipose tissue browning. Cell Mol Life Sci 74:1765-1776
Lee, Hojin; Min, Kisuk; Yi, Jae-Sung et al. (2017) A Phosphoproteomic Screen Identifies a Guanine Nucleotide Exchange Factor for Rab3A Protein as a Mitogen-activated Protein (MAP) Kinase Phosphatase-5-regulated MAP Kinase Target in Interleukin 6 (IL-6) Secretion and Myogenesis. J Biol Chem 292:3581-3590
Giehl, Esther; Lemos, Fernanda O; Huang, Yan et al. (2017) Polycystin 2-dependent cardio-protective mechanisms revealed by cardiac stress. Pflugers Arch :
Khamphaya, Tanaporn; Chukijrungroat, Natsasi; Saengsirisuwan, Vitoon et al. (2017) Nonalcoholic fatty liver disease impairs expression of the type II inositol 1,4,5-trisphosphate receptor. Hepatology :
Lawan, Ahmed; Bennett, Anton M (2017) Mitogen-Activated Protein Kinase Regulation in Hepatic Metabolism. Trends Endocrinol Metab 28:868-878
Zhao, Lin; Feng, Zhihui; Yang, Xiaoyong et al. (2016) The regulatory roles of O-GlcNAcylation in mitochondrial homeostasis and metabolic syndrome. Free Radic Res 50:1080-1088
Wang, Baisheng; Boeckel, Göran R; Huynh, Larry et al. (2016) Neuronal Calcium Sensor 1 Has Two Variants with Distinct Calcium Binding Characteristics. PLoS One 11:e0161414

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