The Cell and Molecular Biology Core (Core B) will provide reagents to assist in the study of nuclear calcium signaling and its effects in liver. The core will generate, purify and maintain adenoviruses and genetically engineered cell lines in which nuclear or cytosolic calcium signaling is suppressed. The core also will assist in the development and characterization of siRNA's requested for use in the individual projects. The core will provide hepatocytes and other cells from MKP-1 knock-out mice and transgenic mice expressing liverspecific expression of nuclear and cytosolic calcium buffering proteins for the analysis of calcium signaling and related studies on growth and metabolism. The core will include dedicated personnel and will be located with the Director's laboratory.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Program Projects (P01)
Project #
5P01DK057751-13
Application #
8463515
Study Section
Special Emphasis Panel (ZDK1-GRB-8)
Project Start
Project End
Budget Start
2013-05-01
Budget End
2014-04-30
Support Year
13
Fiscal Year
2013
Total Cost
$206,267
Indirect Cost
$77,880
Name
Yale University
Department
Type
DUNS #
043207562
City
New Haven
State
CT
Country
United States
Zip Code
06520
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