The Cell and Molecular Biology Core (Core B) will provide reagents to assist in the study of nuclear calcium signaling and its effects in liver. The core will generate, purify and maintain adenoviruses and genetically engineered cell lines in which nuclear or cytosolic calcium signaling is suppressed. The core also will assist in the development and characterization of siRNA's requested for use in the individual projects. The core will provide hepatocytes and other cells from MKP-1 knock-out mice and transgenic mice expressing liverspecific expression of nuclear and cytosolic calcium buffering proteins for the analysis of calcium signaling and related studies on growth and metabolism. The core will include dedicated personnel and will be located with the Director's laboratory.

National Institute of Health (NIH)
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Research Program Projects (P01)
Project #
Application #
Study Section
Special Emphasis Panel (ZDK1-GRB-8)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Yale University
New Haven
United States
Zip Code
Singh, Jay Prakash; Zhang, Kaisi; Wu, Jing et al. (2015) O-GlcNAc signaling in cancer metabolism and epigenetics. Cancer Lett 356:244-50
Lee, Hojin; Yi, Jae-Sung; Lawan, Ahmed et al. (2015) Mining the function of protein tyrosine phosphatases in health and disease. Semin Cell Dev Biol 37:66-72
Kuo, Ivana Y; Kwaczala, Andrea T; Nguyen, Lily et al. (2014) Decreased polycystin 2 expression alters calcium-contraction coupling and changes ?-adrenergic signaling pathways. Proc Natl Acad Sci U S A 111:16604-9
Kuo, Ivana Y; DesRochers, Teresa M; Kimmerling, Erica P et al. (2014) Cyst formation following disruption of intracellular calcium signaling. Proc Natl Acad Sci U S A 111:14283-8
Wu, Jing; Bowe, Damon B; Sadlonova, Andrea et al. (2014) O-GlcNAc transferase is critical for transducin-like enhancer of split (TLE)-mediated repression of canonical Wnt signaling. J Biol Chem 289:12168-76
Amaya, Maria J; Oliveira, Andre G; Guimaraes, Erika S et al. (2014) The insulin receptor translocates to the nucleus to regulate cell proliferation in liver. Hepatology 59:274-83
Chen, Jianxin; Wong, Serena; Nathanson, Michael H et al. (2014) Evaluation of Barrett esophagus by multiphoton microscopy. Arch Pathol Lab Med 138:204-12
Amaya, Maria Jimena; Nathanson, Michael H (2014) Calcium signaling and the secretory activity of bile duct epithelia. Cell Calcium 55:317-24
Li, Min-Dian; Ruan, Hai-Bin; Hughes, Michael E et al. (2013) O-GlcNAc signaling entrains the circadian clock by inhibiting BMAL1/CLOCK ubiquitination. Cell Metab 17:303-10
Amaya, Maria Jimena; Nathanson, Michael H (2013) Calcium signaling in the liver. Compr Physiol 3:515-39

Showing the most recent 10 out of 66 publications