The animal management required for, and the vast amount of data generated from integrative longitudinal studies of the pathophysiology of gastric motor function necessitates considerable organization. The Physiological Characterization and Data Management Core C will provide the infrastructure required to support integrative longitudinal animal studies. Central to this infrastructure is a database, the Electronic Animal Research Record (EARR), designed specifically for this Program Project, that will be managed by Core C. EARR will store data for individual mice on secure servers. Stored data will include measurements and analyses directly made by Core C as well as relevant data generated from each Project and analysis of image datasets made by Imaging Core B. Image datasets will be stored on servers maintained in Imaging Core B but will be searchable from within EARR via a data pipeline between servers. All data will be relationally linked to an individual animal's EARR. Data analysis tools were designed specifically to meet the needs of Project investigators and will provide information required for rapid public sharing of the results of studies proposed in this application. Data mining tools developed by Core C and Imaging Core B will allow researchers to compile data from a vast number of animals to perform retrospective analyses. This database will be flexible to meet the evolving needs of the Project investigators. A second function of Core C will be to perform the gastric emptying assays in mice required by the Projects of the Program Project. The centralized organization of this single assay of gastric motor function will make efficient use of the equipment needed by all Projects, standardize the assay and ensure that quality controls are performed. Core C will also provide support for animal model management to the Projects through training and back-up technical service. Collectively, these functions of Core C will facilitate the Projects to reach their respective research aims. All Projects are united in the overall objective to identify molecular mechanisms of gastric motor dysfunction that will lead to clinical trials that test novel therapeutic interventions. The Physiological Characterization and Data Management Core C will help to make sure this objective is reached.

Public Health Relevance

Physiological Characterization and Data Management Core (Core C) will provide Project investigators a structured, searchable and shareable database to aid the discovery of causes of gastric motor dysfunction with the intent that these discoveries may lead to clinical trials that test new treatments. Core C will also perform non-invasive gastric emptying assays to characterize gastric motor function and support all aspects of animal models management.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Program Projects (P01)
Project #
5P01DK068055-10
Application #
8685959
Study Section
Special Emphasis Panel (ZDK1-GRB-9)
Project Start
Project End
Budget Start
2014-07-01
Budget End
2015-06-30
Support Year
10
Fiscal Year
2014
Total Cost
$100,786
Indirect Cost
$36,878
Name
Mayo Clinic, Rochester
Department
Type
DUNS #
006471700
City
Rochester
State
MN
Country
United States
Zip Code
55905
Rajan, Elizabeth; Al-Bawardy, Badr; Gostout, Christopher J et al. (2018) Endoscopic muscle biopsy sampling of the duodenum and rectum: a pilot survival study in a porcine model to detect myenteric neurons. Gastrointest Endosc 87:600-606
Cipriani, Gianluca; Gibbons, Simon J; Miller, Katie E et al. (2018) Change in Populations of Macrophages Promotes Development of Delayed Gastric Emptying in Mice. Gastroenterology 154:2122-2136.e12
Desai, A; O'Connor, M; Neja, B et al. (2018) Reproducibility of gastric emptying assessed with scintigraphy in patients with upper GI symptoms. Neurogastroenterol Motil 30:e13365
Miller, K E; Bajzer, Ž; Hein, S S et al. (2018) High temporal resolution gastric emptying breath tests in mice. Neurogastroenterol Motil :e13333
Karakashev, Sergey; Zhu, Hengrui; Wu, Shuai et al. (2018) CARM1-expressing ovarian cancer depends on the histone methyltransferase EZH2 activity. Nat Commun 9:631
Zhong, Jian; Ye, Zhenqing; Lenz, Samuel W et al. (2017) Purification of nanogram-range immunoprecipitated DNA in ChIP-seq application. BMC Genomics 18:985
Parthasarathy, Gopanandan; Kudva, Yogish C; Low, Phillip A et al. (2017) Relationship Between Gastric Emptying and Diurnal Glycemic Control in Type 1 Diabetes Mellitus: A Randomized Trial. J Clin Endocrinol Metab 102:398-406
Gibbons, Simon J; Grover, Madhusudan; Choi, Kyoung Moo et al. (2017) Repeat polymorphisms in the Homo sapiens heme oxygenase-1 gene in diabetic and idiopathic gastroparesis. PLoS One 12:e0187772
Hayashi, Yujiro; Toyomasu, Yoshitaka; Saravanaperumal, Siva Arumugam et al. (2017) Hyperglycemia Increases Interstitial Cells of Cajal via MAPK1 and MAPK3 Signaling to ETV1 and KIT, Leading to Rapid Gastric Emptying. Gastroenterology 153:521-535.e20
Camilleri, Michael; McCallum, Richard W; Tack, Jan et al. (2017) Efficacy and Safety of Relamorelin in Diabetics With Symptoms of Gastroparesis: A Randomized, Placebo-Controlled Study. Gastroenterology 153:1240-1250.e2

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