Animal Model Core B will provide all mice used in Projects 1, 2, and 3. The Core will be responsible for ordering mice, performing various manipulations required to induce experimental colitis, maintaining a specific pathogen-free barrier facility, performing roufine surveys, to ensure absence of pathogens in the colony, and assist in harvesting tissues from mice. Centralization of these functions in an Animal Model Core provides uniformity and quality control of mice being studied in the various projects, reduces cost, and will help conserve numbers of mice required by ensuring their optimal use in the projects.
A second aim of the Animal Model Core is that of centralized pathologic analyses which will be performed by Dr. Trenton Schoeb, an expert veterinary pathologist. Tissue sections from the various projects are coded prior to interpretation and quantitative scoring where needed. This facilitates interactions among the projects and also the comparison of results between them. A third purpose of the Animal Model Core is to provide genetically modified transgenic and knockout mouse strains for use in experiments in the various projects. All of the above Aims have been accomplished in the previous cycle. In regard to the third Aim, during the previous cycle a number of novel genetically-modified mouse lines have been generated in Projects 1, 2, and 3 that have been extremely valuable in addressing innate and adaptive immunity to the microbiota, which is the central goal of this Program Project. These mouse lines include a CBirl flagellin T cell receptor transgenic mouse, an IL-10/Thy1.1 reporter mouse (lOBiT), an IL-17F/Thy1.1 reporter mouse, and an IFNy/Thyl.l reporter mouse. These mouse lines will be housed in Core B and made available to the different projects in this renewal application. Dr. Charies Elson will serve as Director and Dr. Casey Weaver will serve as Co- Director in this Core. Dr. Sam Cartner from the Department of Comparative Medicine will serve as Consultant. An Oversight Committee consisting of the PI of each Project plus Drs. Schoeb and Cartner will oversee the usage and function of the Core.

Public Health Relevance

Inflammatory Bowel Disease afflicts 1.4 million Americans. Experimental mouse models of IBD have contributed great insights into the abnormal immune response to the microbiota that results in IBD. This Core will provide genetically-modified mice to the Projects in support of studies on the fundamental mechanisms of IBD.

National Institute of Health (NIH)
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Research Program Projects (P01)
Project #
Application #
Study Section
Special Emphasis Panel (ZDK1-GRB-7)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of Alabama Birmingham
United States
Zip Code
Balasubramani, Anand; Winstead, Colleen J; Turner, Henrietta et al. (2014) Deletion of a conserved cis-element in the Ifng locus highlights the role of acute histone acetylation in modulating inducible gene transcription. PLoS Genet 10:e1003969
Wang, Yan; Godec, Jernej; Ben-Aissa, Khadija et al. (2014) The transcription factors T-bet and Runx are required for the ontogeny of pathogenic interferon-?-producing T helper 17 cells. Immunity 40:355-66
Wolf, Kyle J; Daft, Joseph G; Tanner, Scott M et al. (2014) Consumption of acidic water alters the gut microbiome and decreases the risk of diabetes in NOD mice. J Histochem Cytochem 62:237-50
Alexander, Katie L; Targan, Stephan R; Elson 3rd, Charles O (2014) Microbiota activation and regulation of innate and adaptive immunity. Immunol Rev 260:206-20
Cao, Anthony T; Yao, Suxia; Stefka, Andrew T et al. (2014) TLR4 regulates IFN-? and IL-17 production by both thymic and induced Foxp3+ Tregs during intestinal inflammation. J Leukoc Biol 96:895-905
Staley, Elizabeth M; Tanner, Scott M; Daft, Joseph G et al. (2013) Maintenance of host leukocytes in peripheral immune compartments following lethal irradiation and bone marrow reconstitution: implications for graft versus host disease. Transpl Immunol 28:112-9
Basu, Rajatava; Hatton, Robin D; Weaver, Casey T (2013) The Th17 family: flexibility follows function. Immunol Rev 252:89-103
Viaud, Sophie; Saccheri, Fabiana; Mignot, Gregoire et al. (2013) The intestinal microbiota modulates the anticancer immune effects of cyclophosphamide. Science 342:971-6
Zindl, Carlene L; Lai, Jen-Feng; Lee, Yun Kyung et al. (2013) IL-22-producing neutrophils contribute to antimicrobial defense and restitution of colonic epithelial integrity during colitis. Proc Natl Acad Sci U S A 110:12768-73
Hepworth, Matthew R; Monticelli, Laurel A; Fung, Thomas C et al. (2013) Innate lymphoid cells regulate CD4+ T-cell responses to intestinal commensal bacteria. Nature 498:113-7

Showing the most recent 10 out of 50 publications