Abstract

The Cell Culture/Adenovirus and Lentivirus Preparation Core functions as a """"""""Service Core"""""""" in this PPG.
The specific aims of this service core are to: 1. Grow and maintain cell lines to be used by multiple investigators. 2. Keep frozen stocks of cells and retrieve cells from liquid N2 as requested by Core users. 3. Prepare media, seed, maintain and manipulate cells according to experimental protocols as requested by Core users. 4. Keep computerized list of all cells maintained by the Core. 5. Assign costs to PPG investigators for use of Core resources (provisions of cells. Adenovirus and Lentivirus preparations, etc) to be charged to Core A. 6. Provide small amounts of cultured cells to other investigators both within and outside of Hopkins for pilot experiments (charge-back to be used). 7. Provide cells/reagents (antibodies and cDNAs) of PPG investigators to scientists around Hopkins and outside Hopkins. 8. Train investigators in using epithelial cells in nature;train/advise personnel of PPG investigators concerning use of the Core facility. 9. Provide Adenovirus and Lentivirus construction, growth, purification and titering on basis of pay-for-service to PPG investigators.

Public Health Relevance

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Program Projects (P01)
Project #
5P01DK072084-08
Application #
8543710
Study Section
Special Emphasis Panel (ZDK1-GRB-8)
Project Start
Project End
Budget Start
2013-09-01
Budget End
2014-08-31
Support Year
8
Fiscal Year
2013
Total Cost
$101,778
Indirect Cost
$39,717

  Institution

Name
Johns Hopkins University
Department
Type
DUNS #
001910777
City
Baltimore
State
MD
Country
United States
Zip Code
21218

  Publications

Singh, Varsha; Yang, Jianbo; Yin, Jianyi et al. (2018) Cholera toxin inhibits SNX27-retromer-mediated delivery of cargo proteins to the plasma membrane. J Cell Sci 131:
Yin, Jianyi; Tse, Chung-Ming; Avula, Leela Rani et al. (2018) Molecular Basis and Differentiation-Associated Alterations of Anion Secretion in Human Duodenal Enteroid Monolayers. Cell Mol Gastroenterol Hepatol 5:591-609
Sarker, Rafiquel; Cha, Boyoung; Kovbasnjuk, Olga et al. (2017) Phosphorylation of NHE3-S719 regulates NHE3 activity through the formation of multiple signaling complexes. Mol Biol Cell 28:1754-1767
Qiang, Xiaoling; Liotta, Anthony S; Shiloach, Joseph et al. (2017) New melanocortin-like peptide of E. coli can suppress inflammation via the mammalian melanocortin-1 receptor (MC1R): possible endocrine-like function for microbes of the gut. NPJ Biofilms Microbiomes 3:31
Cha, Boyoung; Yang, Jianbo; Singh, Varsha et al. (2017) PDZ domain-dependent regulation of NHE3 protein by both internal Class II and C-terminal Class I PDZ-binding motifs. J Biol Chem 292:8279-8290
Yu, Huimin; Hasan, Nesrin M; In, Julie G et al. (2017) The Contributions of Human Mini-Intestines to the Study of Intestinal Physiology and Pathophysiology. Annu Rev Physiol 79:291-312
Foulke-Abel, Jennifer; In, Julie; Yin, Jianyi et al. (2016) Human Enteroids as a Model of Upper Small Intestinal Ion Transport Physiology and Pathophysiology. Gastroenterology 150:638-649.e8
Gupta, Arnab; Schell, Michael J; Bhattacharjee, Ashima et al. (2016) Myosin Vb mediates Cu+ export in polarized hepatocytes. J Cell Sci 129:1179-89
Zachos, Nicholas C; Kovbasnjuk, Olga; Foulke-Abel, Jennifer et al. (2016) Human Enteroids/Colonoids and Intestinal Organoids Functionally Recapitulate Normal Intestinal Physiology and Pathophysiology. J Biol Chem 291:3759-66
Cebotaru, Liudmila; Liu, Qiangni; Yanda, Murali K et al. (2016) Inhibition of histone deacetylase 6 activity reduces cyst growth in polycystic kidney disease. Kidney Int 90:90-9

Showing the most recent 10 out of 67 publications