The Cell Culture/Adenovirus and Lentivirus Preparation Core functions as a "Service Core" in this PPG.
The specific aims of this service core are to: 1. Grow and maintain cell lines to be used by multiple investigators. 2. Keep frozen stocks of cells and retrieve cells from liquid N2 as requested by Core users. 3. Prepare media, seed, maintain and manipulate cells according to experimental protocols as requested by Core users. 4. Keep computerized list of all cells maintained by the Core. 5. Assign costs to PPG investigators for use of Core resources (provisions of cells. Adenovirus and Lentivirus preparations, etc) to be charged to Core A. 6. Provide small amounts of cultured cells to other investigators both within and outside of Hopkins for pilot experiments (charge-back to be used). 7. Provide cells/reagents (antibodies and cDNAs) of PPG investigators to scientists around Hopkins and outside Hopkins. 8. Train investigators in using epithelial cells in nature;train/advise personnel of PPG investigators concerning use of the Core facility. 9. Provide Adenovirus and Lentivirus construction, growth, purification and titering on basis of pay-for-service to PPG investigators.
|Cha, Boyoung; Chen, Tiane; Sarker, Rafiquel et al. (2014) Lysophosphatidic acid stimulation of NHE3 exocytosis in polarized epithelial cells occurs with release from NHERF2 via ERK-PLC-PKC? signaling. Am J Physiol Cell Physiol 307:C55-65|
|Braiterman, Lelita T; Murthy, Amrutha; Jayakanthan, Samuel et al. (2014) Distinct phenotype of a Wilson disease mutation reveals a novel trafficking determinant in the copper transporter ATP7B. Proc Natl Acad Sci U S A 111:E1364-73|
|Nyasae, Lydia K; Schell, Michael J; Hubbard, Ann L (2014) Copper directs ATP7B to the apical domain of hepatic cells via basolateral endosomes. Traffic 15:1344-65|
|Yang, Jianbo; Singh, Varsha; Chen, Tian-E et al. (2014) NHERF2/NHERF3 protein heterodimerization and macrocomplex formation are required for the inhibition of NHE3 activity by carbachol. J Biol Chem 289:20039-53|
|Singh, Varsha; Yang, Jianbo; Chen, Tiane-e et al. (2014) Translating molecular physiology of intestinal transport into pharmacologic treatment of diarrhea: stimulation of Na+ absorption. Clin Gastroenterol Hepatol 12:27-31|
|Li, Xuhang; Donowitz, Mark (2014) Fractionation of subcellular membrane vesicles of epithelial and non-epithelial cells by OptiPrep™ density gradient ultracentrifugation. Methods Mol Biol 1174:85-99|
|Singh, Varsha; Lin, Rong; Yang, Jianbo et al. (2014) AKT and GSK-3 are necessary for direct ezrin binding to NHE3 as part of a C-terminal stimulatory complex: role of a novel Ser-rich NHE3 C-terminal motif in NHE3 activity and trafficking. J Biol Chem 289:5449-61|
|Chen, Tiane; Hubbard, Ann; Murtazina, Rakhilya et al. (2014) Myosin VI mediates the movement of NHE3 down the microvillus in intestinal epithelial cells. J Cell Sci 127:3535-45|
|Foulke-Abel, Jennifer; In, Julie; Kovbasnjuk, Olga et al. (2014) Human enteroids as an ex-vivo model of host-pathogen interactions in the gastrointestinal tract. Exp Biol Med (Maywood) 239:1124-34|
|Cheng, Jie; Guggino, William (2013) Ubiquitination and degradation of CFTR by the E3 ubiquitin ligase MARCH2 through its association with adaptor proteins CAL and STX6. PLoS One 8:e68001|
Showing the most recent 10 out of 44 publications