The Mount Sinai Hospital performs approximately 800 intestinal resections every year, constituting a potentially vast resource of tissues for basic investigation. For many years, the P.I. of the PPG (Dr. Mayer) has been working closely with Dr. Harpaz (Director of Gl Pathology) to secure surgical specimens for research, as evidenced by the Preliminary Results presented. Over the past granting period this facilitated the successful completion of human studies for this project demonstrating the success of more formally organizing a Pathology Core that simultaneously supports all projects in the current Program Project Grant. This resource is quite unique as it ensures integration across the several projects such that the same specimen is used for several different types of experiments to permit more meaningful interpretation of data.
The Specific Aims of the Pathology Core are as follows:
Specific Aim 1 : To retrieve, process, and distribute suitable specimens for the four Projects as needed (human - projects 1-4;murine - projects 1-4).
Specific Aim 2 : To stabilize and preserve undistributed tissues for long-term storage and make them available to Projects or investigators as needed.
Specific Aim 3 : To maintain a detailed tissue database documenting the anatomic sources, the gross and microscopic pathological characteristics of the individual tissue specimens, and the clinical and pathological data pertaining to the patients from whom specimens were derived.
Specific Aim 4 : To perform blinded histologic evaluation and scoring of inflammation in the different murine models used in all projects. Immunohistochemistry, and RNA analysis is performed in the specific laboratories of the individual investigators according to their expertise (see below for specifics). Only specimen acquisition, cell isolation (accessioning tech), distribution, storage, histologic analysis and maintenance of the database is performed in this core. The budget reflects the costs of these latter functions.

Public Health Relevance

The Pathology core has been instrumental in providing valuable resources for all investigators, be it human tissues that are well characterized or clear insights into the pathology in a number of new murine models of disease. It is the enabling core for this program.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Program Projects (P01)
Project #
5P01DK072201-09
Application #
8730612
Study Section
Special Emphasis Panel (ZDK1-GRB-8)
Project Start
Project End
Budget Start
2014-09-01
Budget End
2015-08-31
Support Year
9
Fiscal Year
2014
Total Cost
$199,088
Indirect Cost
$81,632
Name
Icahn School of Medicine at Mount Sinai
Department
Type
DUNS #
078861598
City
New York
State
NY
Country
United States
Zip Code
10029
Blander, J Magarian (2016) The comings and goings of MHC class I molecules herald a new dawn in cross-presentation. Immunol Rev 272:65-79
Blander, J Magarian (2016) Death in the intestinal epithelium-basic biology and implications for inflammatory bowel disease. FEBS J 283:2720-30
Campisi, Laura; Barbet, Gaetan; Ding, Yi et al. (2016) Apoptosis in response to microbial infection induces autoreactive TH17 cells. Nat Immunol 17:1084-92
Parkunan, Salai Madhumathi; Randall, C Blake; Astley, Roger A et al. (2016) CXCL1, but not IL-6, significantly impacts intraocular inflammation during infection. J Leukoc Biol 100:1125-1134
Legarda, Diana; Justus, Scott J; Ang, Rosalind L et al. (2016) CYLD Proteolysis Protects Macrophages from TNF-Mediated Auto-necroptosis Induced by LPS and Licensed by Type I IFN. Cell Rep 15:2449-61
Ting, Adrian T; Bertrand, Mathieu J M (2016) More to Life than NF-κB in TNFR1 Signaling. Trends Immunol 37:535-45
Roulis, M; Bongers, G; Armaka, M et al. (2016) Host and microbiota interactions are critical for development of murine Crohn's-like ileitis. Mucosal Immunol 9:787-97
Wang, Juan; Peng, Liang; Zhang, Ruihua et al. (2016) 5-Fluorouracil targets thymidylate synthase in the selective suppression of TH17 cell differentiation. Oncotarget 7:19312-26
Dong, Xiaonan; Cheng, Adam; Zou, Zhongju et al. (2016) Endolysosomal trafficking of viral G protein-coupled receptor functions in innate immunity and control of viral oncogenesis. Proc Natl Acad Sci U S A 113:2994-9
Disteldorf, Erik M; Krebs, Christian F; Paust, Hans-Joachim et al. (2015) CXCL5 drives neutrophil recruitment in TH17-mediated GN. J Am Soc Nephrol 26:55-66

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