CORE A - Optical mapping is a key methodology to be used throughout the program by all the projects. It will allow us to measure features of spinal cord injury-induced lower urinary tract pathology not obtainable by other approaches. These include functional measurement of neuropeptide release by afferent nerves, neural remodeling in the bladder and spinal cord and cell-cell interactions in bladder wall cross-sections and in-line primary cells. Therefore, the Imaging Core A, providing the support to set up and run experiments, will be central to the program. Co-directors of the core, Drs. Kanai and Salama, are experts in optical mapping using voltage- and Ca2+-sensitive dyes with a broad experience in application of this technique to study visceral organs, especially urinary bladder. They will provide three fully functional dual-imaging systems to measure voltage and Ca2+ signals from organs, tissues and cells in the bladder and spinal cord. Two of these are low resolution (256 pixels) fast acquisition (4000 frames/s) PDA systems that can record for minutes. The other is a high resolution (2.7 millon pixels) slow acquisition (540 frames/s) system that can record for seconds. The core will also offer commerically available as well as unique dyes (e.g., PGH-1 to PHG-Vl;developed by Drs. Guy Salama and Allen Waggoner) to measure membrane potential, intracellular Ca2+, K+ and nitric oxide, in vitro or in vivo. The core personnel will also offer expertise in dye delivery and acquisition using unique in-house software. This imaging apporach will utilize the genetically encoded Ca2+ indicator, GCaMP4, expressed through viral vectors, to selectively label sensory and motoneurons and their terminals. Thermoregulated ceramic chambers, custom-built by the core, will be available for recordings from the different preparations. Custom modifications to equipment and software will be provided as required. The core will coordinate the efficient use of imaging systems, assist with setting up experiments, analyzing data and troubleshooting. Imaging Core A will closely interact with Animal Core B for more efficient use of imaging systems and animals, taking into consideration the timepoints for treated animals and correlating them with the needs of individual projects.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Program Projects (P01)
Project #
1P01DK093424-01A1
Application #
8463700
Study Section
Special Emphasis Panel (ZDK1-GRB-6 (O1))
Project Start
Project End
Budget Start
2013-08-20
Budget End
2014-07-31
Support Year
1
Fiscal Year
2013
Total Cost
$298,784
Indirect Cost
$80,966
Name
University of Pittsburgh
Department
Type
DUNS #
004514360
City
Pittsburgh
State
PA
Country
United States
Zip Code
15213
Kullmann, F Aura; Beckel, Jonathan M; McDonnell, Bronagh et al. (2018) Involvement of TRPM4 in detrusor overactivity following spinal cord transection in mice. Naunyn Schmiedebergs Arch Pharmacol 391:1191-1202
Zabbarova, Irina V; Ikeda, Youko; Carder, Evan J et al. (2018) Targeting p75 neurotrophin receptors ameliorates spinal cord injury-induced detrusor sphincter dyssynergia in mice. Neurourol Urodyn 37:2452-2461
Shimizu, Nobutaka; Wada, Naoki; Shimizu, Takahiro et al. (2018) Effects of nerve growth factor neutralization on TRP channel expression in laser-captured bladder afferent neurons in mice with spinal cord injury. Neurosci Lett 683:100-103
Ryu, Jae Cheon; Tooke, Katharine; Malley, Susan E et al. (2018) Role of proNGF/p75 signaling in bladder dysfunction after spinal cord injury. J Clin Invest 128:1772-1786
Wada, Naoki; Shimizu, Takahiro; Shimizu, Nobutaka et al. (2018) The effect of neutralization of nerve growth factor (NGF) on bladder and urethral dysfunction in mice with spinal cord injury. Neurourol Urodyn :
Shimizu, Nobutaka; Doyal, Mark F; Goins, William F et al. (2018) Corrigendum to 'Morphological Changes in Different Populations of Bladder Afferent Neurons Detected by Herpes Simplex Virus (HSV) Vectors with Cell-type-specific Promoters in Mice with Spinal Cord Injury' [Neuroscience 364 (2017) 190-201]. Neuroscience 381:161
Beckel, Jonathan M; de Groat, William C (2018) The effect of the electrophilic fatty acid nitro-oleic acid on TRP channel function in sensory neurons. Nitric Oxide :
Ikeda, Youko; Zabbarova, Irina V; Birder, Lori A et al. (2018) Relaxin-2 therapy reverses radiation-induced fibrosis and restores bladder function in mice. Neurourol Urodyn 37:2441-2451
Shimizu, Takahiro; Majima, Tsuyoshi; Suzuki, Takahisa et al. (2018) Nerve growth factor-dependent hyperexcitability of capsaicin-sensitive bladder afferent neurones in mice with spinal cord injury. Exp Physiol 103:896-904
Kullmann, F A; Chang, H H; Gauthier, C et al. (2018) Serotonergic paraneurones in the female mouse urethral epithelium and their potential role in peripheral sensory information processing. Acta Physiol (Oxf) 222:

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