The Analytical core will provide state-of-the-art analyses that are of high priority to CCEH projects that link chemical exposures with clinical and epidemiological data (Project 1), epigenetic data (Project 2), immunological data (Project 3), and mechanistic data from human neurons (Project 4). Analytical Core will use its extensive experience in analytical chemistry to develop and validate highly sensitive and specific methods to quantitate target compounds in small volumes of plasma from mothers and children, in breast milk, and in urine. Specifically, the Analytical Core will serve center projects by conducting analyses on complex biological samples matrices to identify xenobiotic pollutants that are known or suspected of being developmental neurotoxicants and immunotoxicants, in human epidemiological studies and/or in animal models. Quantitative results will be provided to all CCEH Projects where they will be evaluated in the context of ASD risk, genomic and methylomic interactions, cytokine profiles and their ability to alter Ca^'^-dependent signaling pathways in human neurons and immune cells. The Core's three main objectives are: Objective 1: Provide general analytical support for efficient processing and extraction of small sample volumes archived in the CCEH biobank. Objective 2: Undertake highly sensitive and quantitative analytical chemistry techniques to determine concentrations of polybrominated diphenyl ether (PBDE), polychlorinated biphenyl (PCB), and perfluorinated compounds (PFCs) congener patterns present in maternal serum and pyrethroid pesticide metabolites in urine of women at high risk for giving birth to an autistic child participating in the MARBLES study, and those found in children and their mother participating in CHARGE Objective 3: Develop and implement new analytical techniques to measure. This will include the development of: 3.1. PBDE and PCB congener profiles in the same extracts;and 3.2. Hydroxylated PBDE and PCB (OH-PBDE and OH-PCB) metabolites in the same extracts.
The Analytical Core will provide essential services to all Projects, which all rely on accurate exposure data. This Core will generate the first detailed congener-level assessment of xenobiotic exposures in a wellstudied longitudinal cohort at high risk for autism, accurately quantitate xenobiotics in small volume specimens by modifying extraction and detection methods, and identify lead compounds which will become instrumental to all scientific projects of the Center.
|Fritsch, Erika B; Stegeman, John J; Goldstone, Jared V et al. (2015) Expression and function of ryanodine receptor related pathways in PCB tolerant Atlantic killifish (Fundulus heteroclitus) from New Bedford Harbor, MA, USA. Aquat Toxicol 159:156-66|
|Walker, Cheryl K; Krakowiak, Paula; Baker, Alice et al. (2015) Preeclampsia, placental insufficiency, and autism spectrum disorder or developmental delay. JAMA Pediatr 169:154-62|
|Harrington, Rebecca A; Lee, Li-Ching; Crum, Rosa M et al. (2014) Prenatal SSRI use and offspring with autism spectrum disorder or developmental delay. Pediatrics 133:e1241-8|
|Keil, Alexander P; Daniels, Julie L; Hertz-Picciotto, Irva (2014) Autism spectrum disorder, flea and tick medication, and adjustments for exposure misclassification: the CHARGE (CHildhood Autism Risks from Genetics and Environment) case-control study. Environ Health 13:3|
|Shelton, Janie F; Geraghty, Estella M; Tancredi, Daniel J et al. (2014) Neurodevelopmental disorders and prenatal residential proximity to agricultural pesticides: the CHARGE study. Environ Health Perspect 122:1103-9|
|Piras, I S; Haapanen, L; Napolioni, V et al. (2014) Anti-brain antibodies are associated with more severe cognitive and behavioral profiles in Italian children with Autism Spectrum Disorder. Brain Behav Immun 38:91-9|
|Cao, Zhengyu; Cui, Yanjun; Nguyen, Hai M et al. (2014) Nanomolar bifenthrin alters synchronous Ca2+ oscillations and cortical neuron development independent of sodium channel activity. Mol Pharmacol 85:630-9|
|Medici, Valentina; Shibata, Noreene M; Kharbanda, Kusum K et al. (2014) Maternal choline modifies fetal liver copper, gene expression, DNA methylation, and neonatal growth in the tx-j mouse model of Wilson disease. Epigenetics 9:286-96|
|Akins, Roger S; Krakowiak, Paula; Angkustsiri, Kathleen et al. (2014) Utilization patterns of conventional and complementary/alternative treatments in children with autism spectrum disorders and developmental disabilities in a population-based study. J Dev Behav Pediatr 35:1-10|
|Pretto, Dalyir I; Kumar, Madhur; Cao, Zhengyu et al. (2014) Reduced excitatory amino acid transporter 1 and metabotropic glutamate receptor 5 expression in the cerebellum of fragile X mental retardation gene 1 premutation carriers with fragile X-associated tremor/ataxia syndrome. Neurobiol Aging 35:1189-97|
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