The Facility Core for Small Molecule Mass Spectrometry (SMMS) is integral to the Center's research mission by enabling high-resolution detection and isolation of halogenated organic compounds (HQCs). This Core does not replicate any analytical services or facilities currently available at the Scripps Institution of Oceanography (SIO). The SMMS Facility Core will be directed by P3 Leader Aluwihare, and technical oversight for the facility will be provided Co-Director Dr. Yongxuan Su. Daily operation and routine maintenance will be the responsibility of Mr. Matthew Woolery. The facility will include several uniquely configured gas chromatography-mass spectrometry (GC-MS) instruments that are intimately integrated with the Center's research mission to identify the suite of HOCs accumulating in the marine environment, discover new HOCs of concern in wildlife and humans, and evaluate environmental sources of HOCs. The overall goal of PI is to identify organisms and habitats of interest with respect to HOC production in the Southern California Bight. A GC Electron Capture Detector (|JECD)/MS capable of screening these samples for relevant HOC production will be available through the Core. Project 2 aims to identify and characterize the prevalence of polybrominated HOC biosynthetic pathways in the marine environment. As such, P2 will rely on the same instrumentation to screen cultures and insure that organisms under investigation are producing compounds of interest. The overall goalof P3 is to identify the suite of small, natural HOCs available to enter human populations through seafood consumption. This research will be supported by the analytical capabilities of two GCXGC instruments integrated into the proposed Facility. The >200 HOCs that bioaccumulate in apex predators and humans cannot be adequately separated from co-eluting compounds for definitive identification and quantification without comprehensive GCXGC separation. To establish the biogenic origin of HOCs of interest, P3 will also conduct stable isotope studies on pure compounds that are isolated from apex predators, purified and concentrated for isotope measurement. A uniquely configured, preparative GCXGC instrument that is included in the Facility will support this research. Two liquid chromatography-mass spectrometry (LC-MS) instruments will also be housed in the Core to analyze fermentation extracts and synthetic organic preparations. The Internal Advisory Committee will discuss Facility business during monthly meetings, and the External Advisory Committee will evaluate the Facility Core for its performance annually.

Agency
National Institute of Health (NIH)
Institute
National Institute of Environmental Health Sciences (NIEHS)
Type
Research Program Projects (P01)
Project #
1P01ES021921-01
Application #
8413093
Study Section
Special Emphasis Panel (ZES1-LKB-J (P1))
Project Start
Project End
Budget Start
2012-09-24
Budget End
2013-07-31
Support Year
1
Fiscal Year
2012
Total Cost
$32,228
Indirect Cost
$11,436
Name
University of California San Diego
Department
Type
DUNS #
804355790
City
La Jolla
State
CA
Country
United States
Zip Code
92093
Agarwal, Vinayak; Miles, Zachary D; Winter, Jaclyn M et al. (2017) Enzymatic Halogenation and Dehalogenation Reactions: Pervasive and Mechanistically Diverse. Chem Rev 117:5619-5674
Arakawa, Neal; Aluwihare, Lihini I; Simpson, Andre J et al. (2017) Carotenoids are the likely precursor of a significant fraction of marine dissolved organic matter. Sci Adv 3:e1602976
Agarwal, Vinayak; Blanton, Jessica M; Podell, Sheila et al. (2017) Metagenomic discovery of polybrominated diphenyl ether biosynthesis by marine sponges. Nat Chem Biol 13:537-543
Hong, Mee Young; Hoh, Eunha; Kang, Brian et al. (2017) Fish Oil Contaminated with Persistent Organic Pollutants Induces Colonic Aberrant Crypt Foci Formation and Reduces Antioxidant Enzyme Gene Expression in Rats. J Nutr 147:1524-1530
El Gamal, Abrahim; Agarwal, Vinayak; Diethelm, Stefan et al. (2016) Biosynthesis of coral settlement cue tetrabromopyrrole in marine bacteria by a uniquely adapted brominase-thioesterase enzyme pair. Proc Natl Acad Sci U S A 113:3797-802
Teufel, Robin; Agarwal, Vinayak; Moore, Bradley S (2016) Unusual flavoenzyme catalysis in marine bacteria. Curr Opin Chem Biol 31:31-9
Harvey, Elizabeth L; Deering, Robert W; Rowley, David C et al. (2016) A Bacterial Quorum-Sensing Precursor Induces Mortality in the Marine Coccolithophore, Emiliania huxleyi. Front Microbiol 7:59
El Gamal, Abrahim; Agarwal, Vinayak; Rahman, Imran et al. (2016) Enzymatic Reductive Dehalogenation Controls the Biosynthesis of Marine Bacterial Pyrroles. J Am Chem Soc 138:13167-13170
Hong, Mee Young; Lumibao, Jan; Mistry, Prashila et al. (2015) Fish Oil Contaminated with Persistent Organic Pollutants Reduces Antioxidant Capacity and Induces Oxidative Stress without Affecting Its Capacity to Lower Lipid Concentrations and Systemic Inflammation in Rats. J Nutr 145:939-44
Agarwal, Vinayak; Li, Jie; Rahman, Imran et al. (2015) Complexity of naturally produced polybrominated diphenyl ethers revealed via mass spectrometry. Environ Sci Technol 49:1339-46

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