- CORE The CORE diffraction and computation laboratory provides facilities to all members of the Yale Center for Structural Biology (CSB) for the investigation of macromolecular systems by X-ray crystallography, including computers for data reduction and analysis, solution light scattering equipment and a facility for freezing crystals. Three of the PIs on this application (Steitz, Xiong and Strobel) are major users of this facility and the research projects of one (Breaker) benefit significantly from the light scattering equipment and computers and importantly from the collaboration with the Steitz and Strobel labs on riboswitch structural studies. The staff of the CORE laboratory maintains and upgrades the CSB instrumentation, and assists users with data processing and analysis as well as structure solving. The CORE staff also maintains the CSB website, which is heavily used by structural biologists both inside and outside Yale. This invaluable resource provides its users with access to all the programs and data bases that are generally useful to structural biologists. The programs on the web site are upgraded as new releases appear, and documentation is provided for many of them to further assist users. The CORE laboratory not only provides facilities and staff to assist the CORE lab users with solving their diffraction and computation problems, but importantly it provides the environment and opportunity for the users to interact with and help each other face-to-face. This has proven to be extraordinarily important for learning, problem solving and creative thinking.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Program Projects (P01)
Project #
5P01GM022778-41
Application #
9254571
Study Section
Special Emphasis Panel (ZRG1-BCMB-R)
Project Start
Project End
Budget Start
2017-04-01
Budget End
2018-03-31
Support Year
41
Fiscal Year
2017
Total Cost
$180,734
Indirect Cost
$72,185
Name
Yale University
Department
Type
Domestic Higher Education
DUNS #
043207562
City
New Haven
State
CT
Country
United States
Zip Code
06520
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Sherlock, Madeline E; Sadeeshkumar, Harini; Breaker, Ronald R (2018) Variant Bacterial Riboswitches Associated with Nucleotide Hydrolase Genes Sense Nucleoside Diphosphates. Biochemistry :
Harris, Kimberly A; Zhou, Zhiyuan; Peters, Michelle L et al. (2018) A second RNA-binding protein is essential for ethanol tolerance provided by the bacterial OLE ribonucleoprotein complex. Proc Natl Acad Sci U S A 115:E6319-E6328
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Mirihana Arachchilage, Gayan; Sherlock, Madeline E; Weinberg, Zasha et al. (2018) SAM-VI RNAs selectively bind S-adenosylmethionine and exhibit similarities to SAM-III riboswitches. RNA Biol 15:371-378
Wang, Jimin (2018) Determination of chemical identity and occupancy from experimental density maps. Protein Sci 27:411-420
Sherlock, Madeline E; Sudarsan, Narasimhan; Breaker, Ronald R (2018) Riboswitches for the alarmone ppGpp expand the collection of RNA-based signaling systems. Proc Natl Acad Sci U S A 115:6052-6057
Harris, Kimberly A; Breaker, Ronald R (2018) Large Noncoding RNAs in Bacteria. Microbiol Spectr 6:
Yang, Yang; Kang, Dongwei; Nguyen, Laura A et al. (2018) Structural basis for potent and broad inhibition of HIV-1 RT by thiophene[3,2-d]pyrimidine non-nucleoside inhibitors. Elife 7:
Sherlock, Madeline E; Breaker, Ronald R (2017) Biochemical Validation of a Third Guanidine Riboswitch Class in Bacteria. Biochemistry 56:359-363

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