This Core will carry out five main and distinct activities: peptide synthesis, peptide analysis and characterization, mass spectrometric analysis of synthetic and native peptides, methodological developments as required by synthetic and analytical challenges and discovery projects. Peptide synthesis will be done using the solid phase approach and either Boc or Fmoc strategies. Peptide analysis and characterization will use HPLC, CZE, CD and MS. Methodological developments may include the synthesis of novel amino acids or the testing of novel supports for chromatography, among others. Mass spectrometry will be used independently to follow native conotoxins through their purification steps carried out at the University of Utah and the Salk Institute. Discovery projects will concentrate on the design of novel analogs of selected toxins using the aminoglycine and norcysteine scaffolds in addition to Ala-scans. This Core, by bringing together the synthetic and analytical expertise of an established group will enable members of this Program Project to reach their respective goals both economically and in a timely fashion. The Program Project Director and the PI of this Core set priorities and communicate regularly. Day to day operations will be coordinated by Drs. J. Rivier and W. Fischer at the Salk Institute and Dr. M. Mclntosh at the University of Utah.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Program Projects (P01)
Project #
5P01GM048677-20
Application #
8380812
Study Section
Special Emphasis Panel (ZRG1-MDCN-G)
Project Start
Project End
2014-06-30
Budget Start
2012-07-01
Budget End
2013-06-30
Support Year
20
Fiscal Year
2012
Total Cost
$227,766
Indirect Cost
Name
University of Utah
Department
Type
DUNS #
009095365
City
Salt Lake City
State
UT
Country
United States
Zip Code
84112
Robinson, Samuel D; Li, Qing; Bandyopadhyay, Pradip K et al. (2017) Hormone-like peptides in the venoms of marine cone snails. Gen Comp Endocrinol 244:11-18
Backhaus, Sören; Zakrzewicz, Anna; Richter, Katrin et al. (2017) Surfactant inhibits ATP-induced release of interleukin-1? via nicotinic acetylcholine receptors. J Lipid Res 58:1055-1066
Robinson, Samuel D; Li, Qing; Lu, Aiping et al. (2017) The Venom Repertoire of Conus gloriamaris (Chemnitz, 1777), the Glory of the Sea. Mar Drugs 15:
Amati, Anca-Laura; Zakrzewicz, Anna; Siebers, Kathrin et al. (2017) Chemokines (CCL3, CCL4, and CCL5) Inhibit ATP-Induced Release of IL-1? by Monocytic Cells. Mediators Inflamm 2017:1434872
Hone, Arik J; McIntosh, J Michael (2017) Nicotinic acetylcholine receptors in neuropathic and inflammatory pain. FEBS Lett :
Hone, Arik J; Michael McIntosh, J; Rueda-Ruzafa, Lola et al. (2017) Therapeutic concentrations of varenicline in the presence of nicotine increase action potential firing in human adrenal chromaffin cells. J Neurochem 140:37-52
Romero, Haylie K; Christensen, Sean B; Di Cesare Mannelli, Lorenzo et al. (2017) Inhibition of ?9?10 nicotinic acetylcholine receptors prevents chemotherapy-induced neuropathic pain. Proc Natl Acad Sci U S A 114:E1825-E1832
Memon, Tosifa; Chase, Kevin; Leavitt, Lee S et al. (2017) TRPA1 expression levels and excitability brake by KV channels influence cold sensitivity of TRPA1-expressing neurons. Neuroscience 353:76-86
Li, Qing; Barghi, Neda; Lu, Aiping et al. (2017) Divergence of the Venom Exogene Repertoire in Two Sister Species of Turriconus. Genome Biol Evol 9:2211-2225
Estakhr, Jasem; Abazari, Danya; Frisby, Kaitlyn et al. (2017) Differential Control of Dopaminergic Excitability and Locomotion by Cholinergic Inputs in Mouse Substantia Nigra. Curr Biol 27:1900-1914.e4

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